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Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance
Ma, Xiao-Tong1; He, Xi-Wen1; Li, Wen-You1,2,3,4; Zhang, Yu-Kui1,5
关键词Quartz Crystal Microbalance Glycoproteins 4-mercaptophenylboronic Acid
刊名ANALYTICAL SCIENCES
2016-12-01
32期:12页:1277-1282
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DISTILLATION-PRECIPITATION POLYMERIZATION ; FUNCTIONALIZED MESOPOROUS SILICA ; SURFACE-PLASMON RESONANCE ; MASS-SPECTROMETRY ; AFFINITY-CHROMATOGRAPHY ; MAGNETIC NANOPARTICLES ; MONOLITHIC COLUMN ; CLICK CHEMISTRY ; ENRICHMENT ; GLYCOPEPTIDES
英文摘要Glycosylation plays an important part in many biological processes. However, many glycoproteins are either of low abundance or covered by other components in biological samples. Hence, developing new methods to measure the glycoproteins with both high efficiency and low detection limit is important. In this work, a self-assembled 4-mercaptophenylboronic acid film was coated on a quartz crystal microbalance chip. By optimizing the reaction time and the concentration of 4-mercaptophenylboronic acid, a sensor that specifically responded to glycoproteins was created. Then, several parameters for the prepared sensor were investigated and the working curve for representative glycoprotein-transferrin was established. The linearity range was from 50 to 400 ng/mL and the detection limit was 21.0 ng/mL. The sensor was used to detect transferrin in artificial urine samples. This sensor has a low detection limit of glycoproteins requiring only a small amount of samples, and thus has potential applications in both pharmaceutical and medical areas.
语种英语
WOS记录号WOS:000390975100002
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151855
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
2.State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
3.Tianjin Key Lab Biosensing & Mol Recognit, Tianjin 300071, Peoples R China
4.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,et al. Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance[J]. ANALYTICAL SCIENCES,2016,32(12):1277-1282.
APA Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2016).Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance.ANALYTICAL SCIENCES,32(12),1277-1282.
MLA Ma, Xiao-Tong,et al."Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance".ANALYTICAL SCIENCES 32.12(2016):1277-1282.
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