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题名: Immobilization-free DNA-based homogeneous electrochemical biosensors
作者: Zhang, Fang-Ting1;  Cai, Liang-Yuan1;  Zhou, Ying-Lin1;  Zhang, Xin-Xiang1
关键词: DNA ;  Biosensors ;  Immobilization-free ;  Homogeneous ;  Electrochemistry
刊名: TRAC-TRENDS IN ANALYTICAL CHEMISTRY
发表日期: 2016-12-01
DOI: 10.1016/j.trac.2016.08.012
卷: 85, 页:17-32
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: DNA is an excellent biomaterial for building biosensors utilizing diverse DNA interactions with a broad range of biomolecules and chemical compounds. DNA-based electrochemical biosensors have been successfully used in numerous applications with advantages of rapid detection, high sensitivity and low cost. Typical DNA-based electrochemical biosensors require an immobilization step of DNA probe as the biological recognition element, which always suffers from time-consuming and labor-intensive electrode pretreatment and immobilization process, expensive chemical modification, lower reactivity, and complicated operation. Immobilization-free DNA-based electrochemical biosensors can provide powerful complementary tools and novel design strategies for traditional electrochemical DNA biosensors. They would be advantageous in some ways, such as low cost, easy operation, and time saving. More importantly, the homogeneous reaction could increase the reliability and reproducibility of electrochemical sensor without the immobilization step. This review introduces multiple immobilization-free strategies for developing DNA-based homogeneous electrochemical biosensors, aiming at compensating for the deficiencies of heterogeneous modes. The main signal readout modes and signal enhancement strategies will be discussed separately. Additionally, the burgeoning applications combined with miniaturization of electrochemical devices will be highlighted. (C) 2016 Elsevier B.V. All rights reserved.
关键词[WOS]: ROLLING-CIRCLE AMPLIFICATION ;  HEMIN/G-QUADRUPLEX DNAZYME ;  SINGLE-STRANDED-DNA ;  TARGET RECYCLING AMPLIFICATION ;  HYBRIDIZATION CHAIN-REACTION ;  SEQUENCE-SPECIFIC DETECTION ;  STRUCTURE-SWITCHING DNA ;  REDUCED GRAPHENE OXIDE ;  LABEL-FREE ;  NUCLEIC-ACID
语种: 英语
WOS记录号: WOS:000390737700002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151856
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Minist Educ,Coll Chem, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Fang-Ting,Cai, Liang-Yuan,Zhou, Ying-Lin,et al. Immobilization-free DNA-based homogeneous electrochemical biosensors[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2016,85:17-32.
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