DICP OpenIR
Immobilization-free DNA-based homogeneous electrochemical biosensors
Zhang, Fang-Ting; Cai, Liang-Yuan; Zhou, Ying-Lin; Zhang, Xin-Xiang
KeywordDna Biosensors Immobilization-free Homogeneous Electrochemistry
Source PublicationTRAC-TRENDS IN ANALYTICAL CHEMISTRY
2016-12-01
DOI10.1016/j.trac.2016.08.012
Volume85Pages:17-32
Indexed BySCI
SubtypeReview
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordROLLING-CIRCLE AMPLIFICATION ; HEMIN/G-QUADRUPLEX DNAZYME ; SINGLE-STRANDED-DNA ; TARGET RECYCLING AMPLIFICATION ; HYBRIDIZATION CHAIN-REACTION ; SEQUENCE-SPECIFIC DETECTION ; STRUCTURE-SWITCHING DNA ; REDUCED GRAPHENE OXIDE ; LABEL-FREE ; NUCLEIC-ACID
AbstractDNA is an excellent biomaterial for building biosensors utilizing diverse DNA interactions with a broad range of biomolecules and chemical compounds. DNA-based electrochemical biosensors have been successfully used in numerous applications with advantages of rapid detection, high sensitivity and low cost. Typical DNA-based electrochemical biosensors require an immobilization step of DNA probe as the biological recognition element, which always suffers from time-consuming and labor-intensive electrode pretreatment and immobilization process, expensive chemical modification, lower reactivity, and complicated operation. Immobilization-free DNA-based electrochemical biosensors can provide powerful complementary tools and novel design strategies for traditional electrochemical DNA biosensors. They would be advantageous in some ways, such as low cost, easy operation, and time saving. More importantly, the homogeneous reaction could increase the reliability and reproducibility of electrochemical sensor without the immobilization step. This review introduces multiple immobilization-free strategies for developing DNA-based homogeneous electrochemical biosensors, aiming at compensating for the deficiencies of heterogeneous modes. The main signal readout modes and signal enhancement strategies will be discussed separately. Additionally, the burgeoning applications combined with miniaturization of electrochemical devices will be highlighted. (C) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000390737700002
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151856
Collection中国科学院大连化学物理研究所
AffiliationPeking Univ, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Minist Educ,Coll Chem, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Fang-Ting,Cai, Liang-Yuan,Zhou, Ying-Lin,et al. Immobilization-free DNA-based homogeneous electrochemical biosensors[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2016,85:17-32.
APA Zhang, Fang-Ting,Cai, Liang-Yuan,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2016).Immobilization-free DNA-based homogeneous electrochemical biosensors.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,85,17-32.
MLA Zhang, Fang-Ting,et al."Immobilization-free DNA-based homogeneous electrochemical biosensors".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 85(2016):17-32.
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