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题名: Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography
作者: Yu, Wenyi1, 2;  Jin, Hongli3;  Shen, Aijin3;  Deng, Liang1, 2;  Shi, Jianlian1, 2;  Xue, Xingya3;  Guo, Yadong1, 2;  Liu, Yanfang3;  Liang, Xinmiao3
关键词: Glycyrrhizin ;  Hydrophilic interaction solid phase extraction ;  Licorice ;  Preparative reversed-phase liquid chromatography
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2017
DOI: 10.1016/j.jchromb.2016.11.031
卷: 1040, 页:47-52
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Glycyrrhizin (GA), a major bioactive compound in licorice, has been extensively used throughout the world as a medicine to treat chronic viral hepatitis and allergic dermatitis. In this study, a new method based on hydrophilic interaction solid phase extraction (HILIC-SPE) and preparative reversed-phase liquid chromatography (prep-RPLC) was developed to purify GA with high purity from the complex licorice extract. Via evaluation of retention behavior of GA and flavonoids in different commercially available columns, a hydrophilic column-Click XIon was finally chosen for the purification due to its excellent resolution toward GA and flavonoids under HILIC mode. To optimize the SPE elution conditions, relative factors including water content, pH and ionic strength had been investigated in chromatographic condition. The result indicated that the most appropriate water content was 30% and pH at 4.00, as well as salt concentration should be controlled at 5 mM. In addition, the optimization revealed that GA experiences both hydrophilic interaction and ion-exchange interaction on the Click XIon material. According to the chromatographic evaluation, the optimized conditions were applied to HILIC-SPE to enrich GA from licorice, which leads to an increased content of GA from 13.67% to 64.22%. Finally, prep-RPLC was performed to obtain GA with purity higher than 99.00%, which demonstrating great prospect in large-scale preparation of GA. (C) 2016 Published by Elsevier B.V.
关键词[WOS]: COUNTER-CURRENT CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  SEPARATION ;  ACID ;  LIQUIRITIN ;  MILLENNIA ;  SAPONINS ;  SORBENT ;  SAMPLES ;  GLABRA
语种: 英语
WOS记录号: WOS:000392355000006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151859
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China
2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yu, Wenyi,Jin, Hongli,Shen, Aijin,et al. Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1040:47-52.
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