DICP OpenIR
Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography
Yu, Wenyi1,2; Jin, Hongli3; Shen, Aijin3; Deng, Liang1,2; Shi, Jianlian1,2; Xue, Xingya3; Guo, Yadong1,2; Liu, Yanfang3; Liang, Xinmiao3
关键词Glycyrrhizin Hydrophilic Interaction Solid Phase Extraction Licorice Preparative Reversed-phase Liquid Chromatography
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2017
DOI10.1016/j.jchromb.2016.11.031
1040页:47-52
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]COUNTER-CURRENT CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SEPARATION ; ACID ; LIQUIRITIN ; MILLENNIA ; SAPONINS ; SORBENT ; SAMPLES ; GLABRA
英文摘要Glycyrrhizin (GA), a major bioactive compound in licorice, has been extensively used throughout the world as a medicine to treat chronic viral hepatitis and allergic dermatitis. In this study, a new method based on hydrophilic interaction solid phase extraction (HILIC-SPE) and preparative reversed-phase liquid chromatography (prep-RPLC) was developed to purify GA with high purity from the complex licorice extract. Via evaluation of retention behavior of GA and flavonoids in different commercially available columns, a hydrophilic column-Click XIon was finally chosen for the purification due to its excellent resolution toward GA and flavonoids under HILIC mode. To optimize the SPE elution conditions, relative factors including water content, pH and ionic strength had been investigated in chromatographic condition. The result indicated that the most appropriate water content was 30% and pH at 4.00, as well as salt concentration should be controlled at 5 mM. In addition, the optimization revealed that GA experiences both hydrophilic interaction and ion-exchange interaction on the Click XIon material. According to the chromatographic evaluation, the optimized conditions were applied to HILIC-SPE to enrich GA from licorice, which leads to an increased content of GA from 13.67% to 64.22%. Finally, prep-RPLC was performed to obtain GA with purity higher than 99.00%, which demonstrating great prospect in large-scale preparation of GA. (C) 2016 Published by Elsevier B.V.
语种英语
WOS记录号WOS:000392355000006
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151859
专题中国科学院大连化学物理研究所
作者单位1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China
2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Yu, Wenyi,Jin, Hongli,Shen, Aijin,et al. Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1040:47-52.
APA Yu, Wenyi.,Jin, Hongli.,Shen, Aijin.,Deng, Liang.,Shi, Jianlian.,...&Liang, Xinmiao.(2017).Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1040,47-52.
MLA Yu, Wenyi,et al."Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1040(2017):47-52.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yu, Wenyi]的文章
[Jin, Hongli]的文章
[Shen, Aijin]的文章
百度学术
百度学术中相似的文章
[Yu, Wenyi]的文章
[Jin, Hongli]的文章
[Shen, Aijin]的文章
必应学术
必应学术中相似的文章
[Yu, Wenyi]的文章
[Jin, Hongli]的文章
[Shen, Aijin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。