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Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography
Yu, Wenyi1,2; Jin, Hongli3; Shen, Aijin3; Deng, Liang1,2; Shi, Jianlian1,2; Xue, Xingya3; Guo, Yadong1,2; Liu, Yanfang3; Liang, Xinmiao3
KeywordGlycyrrhizin Hydrophilic Interaction Solid Phase Extraction Licorice Preparative Reversed-phase Liquid Chromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2017
DOI10.1016/j.jchromb.2016.11.031
Volume1040Pages:47-52
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SEPARATION ; ACID ; LIQUIRITIN ; MILLENNIA ; SAPONINS ; SORBENT ; SAMPLES ; GLABRA
AbstractGlycyrrhizin (GA), a major bioactive compound in licorice, has been extensively used throughout the world as a medicine to treat chronic viral hepatitis and allergic dermatitis. In this study, a new method based on hydrophilic interaction solid phase extraction (HILIC-SPE) and preparative reversed-phase liquid chromatography (prep-RPLC) was developed to purify GA with high purity from the complex licorice extract. Via evaluation of retention behavior of GA and flavonoids in different commercially available columns, a hydrophilic column-Click XIon was finally chosen for the purification due to its excellent resolution toward GA and flavonoids under HILIC mode. To optimize the SPE elution conditions, relative factors including water content, pH and ionic strength had been investigated in chromatographic condition. The result indicated that the most appropriate water content was 30% and pH at 4.00, as well as salt concentration should be controlled at 5 mM. In addition, the optimization revealed that GA experiences both hydrophilic interaction and ion-exchange interaction on the Click XIon material. According to the chromatographic evaluation, the optimized conditions were applied to HILIC-SPE to enrich GA from licorice, which leads to an increased content of GA from 13.67% to 64.22%. Finally, prep-RPLC was performed to obtain GA with purity higher than 99.00%, which demonstrating great prospect in large-scale preparation of GA. (C) 2016 Published by Elsevier B.V.
Language英语
WOS IDWOS:000392355000006
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151859
Collection中国科学院大连化学物理研究所
Affiliation1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China
2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yu, Wenyi,Jin, Hongli,Shen, Aijin,et al. Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1040:47-52.
APA Yu, Wenyi.,Jin, Hongli.,Shen, Aijin.,Deng, Liang.,Shi, Jianlian.,...&Liang, Xinmiao.(2017).Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1040,47-52.
MLA Yu, Wenyi,et al."Purification of high-purity glycyrrhizin from licorice using hydrophilic interaction solid phase extraction coupled with preparative reversed-phase liquid chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1040(2017):47-52.
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