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题名: Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography
作者: Yang, Fan2;  Zhao, Manxi3;  Zhang, Xiuli1;  Li, Qiang3;  Xiao, Yuansheng1;  Ke, Xiao3;  He, Jian1;  Ye, Liang3;  Feng, Jiatao1;  Hao, Xiaofeng3;  Ke, Zunhong3;  Liang, Xinmiao1
关键词: Two-dimensional preparative LC ;  Orthogonal ;  RP x HILIC ;  RP x RP ;  Separation strategy
刊名: CHROMATOGRAPHIA
发表日期: 2017
DOI: 10.1007/s10337-016-3218-6
卷: 80, 期:1, 页:39-43
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An off-line orthogonal two-dimensional preparative liquid chromatography method was developed for isolation of high-purity compounds from aqueous extracts of Pinus massoniana Lamb., which has efficient therapeutic properties in recipes of traditional Chinese medicines. A polar-enhanced reversed-phase (RP) column, XAqua C18 was selected for the first-dimensional separation. A HILIC column and a hydrophobic RP column were both explored in the second dimension. The preparation results indicated hydrophilic RP x HILIC mode would provide better separation efficiency for polar complexes, i.e., the second-dimensional separation was finished in less than 30 min and high-purity compounds were obtained. In this work, 16 fractions were collected efficiently in the first dimension with a recovery up to 96%, and six compounds with high purity and enough quantity were identified by NMR and high resolution mass spectrometry, including epicatechin, betuloside, taxifolin-3-O-xyloside, cedrusin-4-O-glucoside, massonianoside C and massonianoside D, and five are glycosides, including flavonoid glycosides, lignanoid glycosides and an arylbutanoid glycoside. Betuloside was first reported in the preparation of Pinus massoniana Lamb. This work demonstrated a flexible strategy of compromise in between time and resolution for selecting solvent and column to enhance preparative efficiency of polar complex systems.
关键词[WOS]: FLAVONOID GLYCOSIDES ;  STATIONARY PHASES ;  IDENTIFICATION ;  NEEDLES
语种: 英语
WOS记录号: WOS:000392315400006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151872
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Serv Ctr, Dalian 116023, Peoples R China
3.Chengdu Kanghong Pharmaceut Ltd Co, Chengdu 610036, Peoples R China

Recommended Citation:
Yang, Fan,Zhao, Manxi,Zhang, Xiuli,et al. Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography[J]. CHROMATOGRAPHIA,2017,80(1):39-43.
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