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Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography
Yang, Fan2; Zhao, Manxi3; Zhang, Xiuli1; Li, Qiang3; Xiao, Yuansheng1; Ke, Xiao3; He, Jian1; Ye, Liang3; Feng, Jiatao1; Hao, Xiaofeng3; Ke, Zunhong3; Liang, Xinmiao1
关键词Two-dimensional Preparative Lc Orthogonal Rp x Hilic Rp x Rp Separation Strategy
刊名CHROMATOGRAPHIA
2017
DOI10.1007/s10337-016-3218-6
80期:1页:39-43
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FLAVONOID GLYCOSIDES ; STATIONARY PHASES ; IDENTIFICATION ; NEEDLES
英文摘要An off-line orthogonal two-dimensional preparative liquid chromatography method was developed for isolation of high-purity compounds from aqueous extracts of Pinus massoniana Lamb., which has efficient therapeutic properties in recipes of traditional Chinese medicines. A polar-enhanced reversed-phase (RP) column, XAqua C18 was selected for the first-dimensional separation. A HILIC column and a hydrophobic RP column were both explored in the second dimension. The preparation results indicated hydrophilic RP x HILIC mode would provide better separation efficiency for polar complexes, i.e., the second-dimensional separation was finished in less than 30 min and high-purity compounds were obtained. In this work, 16 fractions were collected efficiently in the first dimension with a recovery up to 96%, and six compounds with high purity and enough quantity were identified by NMR and high resolution mass spectrometry, including epicatechin, betuloside, taxifolin-3-O-xyloside, cedrusin-4-O-glucoside, massonianoside C and massonianoside D, and five are glycosides, including flavonoid glycosides, lignanoid glycosides and an arylbutanoid glycoside. Betuloside was first reported in the preparation of Pinus massoniana Lamb. This work demonstrated a flexible strategy of compromise in between time and resolution for selecting solvent and column to enhance preparative efficiency of polar complex systems.
语种英语
WOS记录号WOS:000392315400006
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151872
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Serv Ctr, Dalian 116023, Peoples R China
3.Chengdu Kanghong Pharmaceut Ltd Co, Chengdu 610036, Peoples R China
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GB/T 7714
Yang, Fan,Zhao, Manxi,Zhang, Xiuli,et al. Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography[J]. CHROMATOGRAPHIA,2017,80(1):39-43.
APA Yang, Fan.,Zhao, Manxi.,Zhang, Xiuli.,Li, Qiang.,Xiao, Yuansheng.,...&Liang, Xinmiao.(2017).Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography.CHROMATOGRAPHIA,80(1),39-43.
MLA Yang, Fan,et al."Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography".CHROMATOGRAPHIA 80.1(2017):39-43.
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