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Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry
Lu, Congcong1; Tian, Shanshan2; Zhai, Guijin2; Yuan, Zuofei3; Li, Yijun1; He, Xiwen1; Zhang, Yukui1,4; Zhang, Kai1,2
刊名ACS CHEMICAL BIOLOGY
2017
DOI10.1021/acschembio.6b00797
12期:1页:57-62
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]INDUCED STRUCTURAL-CHANGES ; PROTEIN INTERACTIONS ; DNA APTAMER ; STREPTAVIDIN ; ASSEMBLIES ; MOLECULE
英文摘要Histone proteins, which could interact with DNA, play important roles in the regulation of chromatin structures, transcription, and other DNA-based biological processes. Here, we developed a novel aptamer-based probe for the analysis of histone H4-aptamer interfaces. This probe contains a DNA sequence for specific recognition of histone H4, a biotin tag for affinity enrichment, an aryl azide photoactive group for cross-linking and a cleavable disulfide group to dissociate aptamer from labeled histones. We successfully achieved specific enrichment of histone H4 and further developed a new analysis strategy for histone-aptamer interaction by photo cross-linking mass spectrometry. The binding area of histone H4 to aptamer was investigated and discussed for the first time. This strategy exhibits great potential and Might further contribute to the understanding of histone-DNA interaction patterns.
语种英语
WOS记录号WOS:000392569400007
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151884
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet, Key Lab Immune Microenvironm & Dis,Ministry Educ, Tianjin 300070, Peoples R China
3.Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Lu, Congcong,Tian, Shanshan,Zhai, Guijin,et al. Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry[J]. ACS CHEMICAL BIOLOGY,2017,12(1):57-62.
APA Lu, Congcong.,Tian, Shanshan.,Zhai, Guijin.,Yuan, Zuofei.,Li, Yijun.,...&Zhang, Kai.(2017).Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry.ACS CHEMICAL BIOLOGY,12(1),57-62.
MLA Lu, Congcong,et al."Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry".ACS CHEMICAL BIOLOGY 12.1(2017):57-62.
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