DICP OpenIR
Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry
Lu, Congcong1; Tian, Shanshan2; Zhai, Guijin2; Yuan, Zuofei3; Li, Yijun1; He, Xiwen1; Zhang, Yukui1,4; Zhang, Kai1,2
Source PublicationACS CHEMICAL BIOLOGY
2017
DOI10.1021/acschembio.6b00797
Volume12Issue:1Pages:57-62
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordINDUCED STRUCTURAL-CHANGES ; PROTEIN INTERACTIONS ; DNA APTAMER ; STREPTAVIDIN ; ASSEMBLIES ; MOLECULE
AbstractHistone proteins, which could interact with DNA, play important roles in the regulation of chromatin structures, transcription, and other DNA-based biological processes. Here, we developed a novel aptamer-based probe for the analysis of histone H4-aptamer interfaces. This probe contains a DNA sequence for specific recognition of histone H4, a biotin tag for affinity enrichment, an aryl azide photoactive group for cross-linking and a cleavable disulfide group to dissociate aptamer from labeled histones. We successfully achieved specific enrichment of histone H4 and further developed a new analysis strategy for histone-aptamer interaction by photo cross-linking mass spectrometry. The binding area of histone H4 to aptamer was investigated and discussed for the first time. This strategy exhibits great potential and Might further contribute to the understanding of histone-DNA interaction patterns.
Language英语
WOS IDWOS:000392569400007
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151884
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet, Key Lab Immune Microenvironm & Dis,Ministry Educ, Tianjin 300070, Peoples R China
3.Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Lu, Congcong,Tian, Shanshan,Zhai, Guijin,et al. Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry[J]. ACS CHEMICAL BIOLOGY,2017,12(1):57-62.
APA Lu, Congcong.,Tian, Shanshan.,Zhai, Guijin.,Yuan, Zuofei.,Li, Yijun.,...&Zhang, Kai.(2017).Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry.ACS CHEMICAL BIOLOGY,12(1),57-62.
MLA Lu, Congcong,et al."Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry".ACS CHEMICAL BIOLOGY 12.1(2017):57-62.
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