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题名: Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry
作者: Lu, Congcong1;  Tian, Shanshan2;  Zhai, Guijin2;  Yuan, Zuofei3;  Li, Yijun1;  He, Xiwen1;  Zhang, Yukui1, 4;  Zhang, Kai1, 2
刊名: ACS CHEMICAL BIOLOGY
发表日期: 2017
DOI: 10.1021/acschembio.6b00797
卷: 12, 期:1, 页:57-62
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Histone proteins, which could interact with DNA, play important roles in the regulation of chromatin structures, transcription, and other DNA-based biological processes. Here, we developed a novel aptamer-based probe for the analysis of histone H4-aptamer interfaces. This probe contains a DNA sequence for specific recognition of histone H4, a biotin tag for affinity enrichment, an aryl azide photoactive group for cross-linking and a cleavable disulfide group to dissociate aptamer from labeled histones. We successfully achieved specific enrichment of histone H4 and further developed a new analysis strategy for histone-aptamer interaction by photo cross-linking mass spectrometry. The binding area of histone H4 to aptamer was investigated and discussed for the first time. This strategy exhibits great potential and Might further contribute to the understanding of histone-DNA interaction patterns.
关键词[WOS]: INDUCED STRUCTURAL-CHANGES ;  PROTEIN INTERACTIONS ;  DNA APTAMER ;  STREPTAVIDIN ;  ASSEMBLIES ;  MOLECULE
语种: 英语
WOS记录号: WOS:000392569400007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151884
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet, Key Lab Immune Microenvironm & Dis,Ministry Educ, Tianjin 300070, Peoples R China
3.Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Lu, Congcong,Tian, Shanshan,Zhai, Guijin,et al. Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry[J]. ACS CHEMICAL BIOLOGY,2017,12(1):57-62.
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