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题名: A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems
作者: Zou, Li-Wei1, 2;  Wang, Ping1;  Qian, Xing-Kai1;  Feng, Lei3;  Yu, Yang1;  Wang, Dan-Dan1;  Jin, Qiang1;  Hou, Jie3;  Liu, Zhi-Hong4;  Ge, Guang-Bo1;  Yang, Ling2
关键词: Dipeptidyl peptidase IV ;  Two-photon fluorescent probe ;  Cells and tissues imaging ;  Inhibitor screening
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2017-04-15
DOI: 10.1016/j.bios.2016.11.068
卷: 90, 页:283-289
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: In this study, a highly specific ratiometric two-photon fluorescent probe GP-BAN was developed and well characterized to monitor dipeptidyl peptidase IV in plasma and living systems. GP-BAN was designed on the basis of the catalytic properties and substrate preference of DPP-IV, and it could be readily hydrolyzed upon addition of DPP-IV under physiological conditions. Both reaction phenotyping and inhibition assays demonstrated that GP-BAN displayed good reactivity and high selectivity towards DPP-IV over other human serine hydrolases including FAP, DPP-VIII, and DPP-IX. The probe was successfully used to monitor the real activities of DPP-IV in complex biological systems including diluted plasma, while it could be used for high throughput screening of DPP-W inhibitors by using human plasma or tissue preparations as enzyme sources. As a two photon fluorescent probe, GP-BAN was also successfully used for two-photon imaging of endogenous DPP-IV in living cells and tissues, and showed high ratiometric imaging resolution and deep-tissue penetration ability. Taken together, a ratiometric two-photon fluorescent probe GP-BAN was developed and well-characterized for highly selective and sensitive detection of DPP-IV in complex biological systems, which could serve as a promising imaging tool to explore the biological functions and physiological roles of this key enzyme in living systems.
关键词[WOS]: FIBROBLAST ACTIVATION PROTEIN ;  HUMAN CARBOXYLESTERASE 2 ;  IN-VITRO ;  BIOLOGICAL APPLICATIONS ;  CANCER ;  CELLS ;  CD26 ;  ASSOCIATION ;  MICROSCOPY ;  INHIBITORS
语种: 英语
WOS记录号: WOS:000392768000039
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151895
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
3.Dalian Med Univ, Dalian 116044, Peoples R China
4.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Coll Chem & Mol Sci, Wuhan, Peoples R China

Recommended Citation:
Zou, Li-Wei,Wang, Ping,Qian, Xing-Kai,et al. A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems[J]. BIOSENSORS & BIOELECTRONICS,2017,90:283-289.
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