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A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems
Zou, Li-Wei1,2; Wang, Ping1; Qian, Xing-Kai1; Feng, Lei3; Yu, Yang1; Wang, Dan-Dan1; Jin, Qiang1; Hou, Jie3; Liu, Zhi-Hong4; Ge, Guang-Bo1; Yang, Ling2
KeywordDipeptidyl Peptidase Iv Two-photon Fluorescent Probe Cells And Tissues Imaging Inhibitor Screening
Source PublicationBIOSENSORS & BIOELECTRONICS
2017-04-15
DOI10.1016/j.bios.2016.11.068
Volume90Pages:283-289
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordFIBROBLAST ACTIVATION PROTEIN ; HUMAN CARBOXYLESTERASE 2 ; IN-VITRO ; BIOLOGICAL APPLICATIONS ; CANCER ; CELLS ; CD26 ; ASSOCIATION ; MICROSCOPY ; INHIBITORS
AbstractIn this study, a highly specific ratiometric two-photon fluorescent probe GP-BAN was developed and well characterized to monitor dipeptidyl peptidase IV in plasma and living systems. GP-BAN was designed on the basis of the catalytic properties and substrate preference of DPP-IV, and it could be readily hydrolyzed upon addition of DPP-IV under physiological conditions. Both reaction phenotyping and inhibition assays demonstrated that GP-BAN displayed good reactivity and high selectivity towards DPP-IV over other human serine hydrolases including FAP, DPP-VIII, and DPP-IX. The probe was successfully used to monitor the real activities of DPP-IV in complex biological systems including diluted plasma, while it could be used for high throughput screening of DPP-W inhibitors by using human plasma or tissue preparations as enzyme sources. As a two photon fluorescent probe, GP-BAN was also successfully used for two-photon imaging of endogenous DPP-IV in living cells and tissues, and showed high ratiometric imaging resolution and deep-tissue penetration ability. Taken together, a ratiometric two-photon fluorescent probe GP-BAN was developed and well-characterized for highly selective and sensitive detection of DPP-IV in complex biological systems, which could serve as a promising imaging tool to explore the biological functions and physiological roles of this key enzyme in living systems.
Language英语
WOS IDWOS:000392768000039
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151895
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
3.Dalian Med Univ, Dalian 116044, Peoples R China
4.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Coll Chem & Mol Sci, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Zou, Li-Wei,Wang, Ping,Qian, Xing-Kai,et al. A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems[J]. BIOSENSORS & BIOELECTRONICS,2017,90:283-289.
APA Zou, Li-Wei.,Wang, Ping.,Qian, Xing-Kai.,Feng, Lei.,Yu, Yang.,...&Yang, Ling.(2017).A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems.BIOSENSORS & BIOELECTRONICS,90,283-289.
MLA Zou, Li-Wei,et al."A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems".BIOSENSORS & BIOELECTRONICS 90(2017):283-289.
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