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Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice
Gu, Rui1,4; Zhang, Xu2,5; Zhang, Ge3; Tao, Tingting1,2; Yu, Haibo5; Liu, Lianqing5; Dou, Ying1; Li, Aiping1; Qin, Jianhua2
关键词Microfluidic Device Microglia Glioma Epithelial-mesenchymal Transition Phagocytosis Migration
刊名NEUROCHEMICAL RESEARCH
2017-05-01
DOI10.1007/s11064-017-2204-1
42期:5页:1478-1487
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]ACTIVATED MICROGLIA ; CELLS ; TRANSITION ; MATRIX ; MECHANISMS ; MACROPHAGE ; RESISTANT ; MIGRATION ; INVASION ; SYSTEM
英文摘要It has been proven that microglia are involved in both early and late stages of glioma progression and contribute substantially to the tumor mass of gliomas. Because no appropriate in vitro or in vivo investigative approach is available, the dynamic interaction between microglia and gliomas during tumor formation remains unclear. In this study, three types of microfluidic assay were developed to examine the outcomes of the dynamic interaction between microglia and gliomas. Co-migration assay and two-dimensional cell co-culture assay have been used to show that microglial BV-2 cells migrate toward C6 glioma cells and inhibit tumor growth during the early stage of tumorigenesis. However, in three-dimensional cell spheres (three-dimensional cell co-culture assay) that contain a large amount of glioma cells, mimicking the late stage of glioma growth, the phagocytosis of microglia was suppressed, which suggests that glioma cells could reeducate classically activated microglia into a tumor-promoting state at some point during tumor progression. Notably, we found that microglia could contribute to tumor invasion and acquisition of the epithelial-mesenchymal transition phenotype in the glioma microenvironment during the early stage and the late stage of tumor progression. In conclusion, we have developed a potential quantitative method for in vitro study of glioma immunity and provided evidence for the duality of glioma-associated microglia.
语种英语
WOS记录号WOS:000399906000021
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151975
专题中国科学院大连化学物理研究所
作者单位1.Dalian Med Univ, West Sect 9, Dept Physiol, Lvshun South Rd, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Med Univ, West Sect 9, Dept Immunol, Lvshun South Rd, Dalian 116044, Peoples R China
4.Gen Hosp Chengdu Army, Ctr Lab Dept, Chengdu 610083, Peoples R China
5.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China
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Gu, Rui,Zhang, Xu,Zhang, Ge,et al. Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice[J]. NEUROCHEMICAL RESEARCH,2017,42(5):1478-1487.
APA Gu, Rui.,Zhang, Xu.,Zhang, Ge.,Tao, Tingting.,Yu, Haibo.,...&Qin, Jianhua.(2017).Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice.NEUROCHEMICAL RESEARCH,42(5),1478-1487.
MLA Gu, Rui,et al."Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice".NEUROCHEMICAL RESEARCH 42.5(2017):1478-1487.
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