DICP OpenIR
Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice
Gu, Rui1,4; Zhang, Xu2,5; Zhang, Ge3; Tao, Tingting1,2; Yu, Haibo5; Liu, Lianqing5; Dou, Ying1; Li, Aiping1; Qin, Jianhua2
KeywordMicrofluidic Device Microglia Glioma Epithelial-mesenchymal Transition Phagocytosis Migration
Source PublicationNEUROCHEMICAL RESEARCH
2017-05-01
DOI10.1007/s11064-017-2204-1
Volume42Issue:5Pages:1478-1487
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Neurosciences
WOS Research AreaBiochemistry & Molecular Biology ; Neurosciences & Neurology
WOS KeywordACTIVATED MICROGLIA ; CELLS ; TRANSITION ; MATRIX ; MECHANISMS ; MACROPHAGE ; RESISTANT ; MIGRATION ; INVASION ; SYSTEM
AbstractIt has been proven that microglia are involved in both early and late stages of glioma progression and contribute substantially to the tumor mass of gliomas. Because no appropriate in vitro or in vivo investigative approach is available, the dynamic interaction between microglia and gliomas during tumor formation remains unclear. In this study, three types of microfluidic assay were developed to examine the outcomes of the dynamic interaction between microglia and gliomas. Co-migration assay and two-dimensional cell co-culture assay have been used to show that microglial BV-2 cells migrate toward C6 glioma cells and inhibit tumor growth during the early stage of tumorigenesis. However, in three-dimensional cell spheres (three-dimensional cell co-culture assay) that contain a large amount of glioma cells, mimicking the late stage of glioma growth, the phagocytosis of microglia was suppressed, which suggests that glioma cells could reeducate classically activated microglia into a tumor-promoting state at some point during tumor progression. Notably, we found that microglia could contribute to tumor invasion and acquisition of the epithelial-mesenchymal transition phenotype in the glioma microenvironment during the early stage and the late stage of tumor progression. In conclusion, we have developed a potential quantitative method for in vitro study of glioma immunity and provided evidence for the duality of glioma-associated microglia.
Language英语
WOS IDWOS:000399906000021
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151975
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Med Univ, West Sect 9, Dept Physiol, Lvshun South Rd, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Med Univ, West Sect 9, Dept Immunol, Lvshun South Rd, Dalian 116044, Peoples R China
4.Gen Hosp Chengdu Army, Ctr Lab Dept, Chengdu 610083, Peoples R China
5.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China
Recommended Citation
GB/T 7714
Gu, Rui,Zhang, Xu,Zhang, Ge,et al. Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice[J]. NEUROCHEMICAL RESEARCH,2017,42(5):1478-1487.
APA Gu, Rui.,Zhang, Xu.,Zhang, Ge.,Tao, Tingting.,Yu, Haibo.,...&Qin, Jianhua.(2017).Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice.NEUROCHEMICAL RESEARCH,42(5),1478-1487.
MLA Gu, Rui,et al."Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice".NEUROCHEMICAL RESEARCH 42.5(2017):1478-1487.
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