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题名: Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice
作者: Gu, Rui1, 4;  Zhang, Xu2, 5;  Zhang, Ge3;  Tao, Tingting1, 2;  Yu, Haibo5;  Liu, Lianqing5;  Dou, Ying1;  Li, Aiping1;  Qin, Jianhua2
关键词: Microfluidic device ;  Microglia ;  Glioma ;  Epithelial-mesenchymal transition ;  Phagocytosis ;  Migration
刊名: NEUROCHEMICAL RESEARCH
发表日期: 2017-05-01
DOI: 10.1007/s11064-017-2204-1
卷: 42, 期:5, 页:1478-1487
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ;  Neurosciences & Neurology
英文摘要: It has been proven that microglia are involved in both early and late stages of glioma progression and contribute substantially to the tumor mass of gliomas. Because no appropriate in vitro or in vivo investigative approach is available, the dynamic interaction between microglia and gliomas during tumor formation remains unclear. In this study, three types of microfluidic assay were developed to examine the outcomes of the dynamic interaction between microglia and gliomas. Co-migration assay and two-dimensional cell co-culture assay have been used to show that microglial BV-2 cells migrate toward C6 glioma cells and inhibit tumor growth during the early stage of tumorigenesis. However, in three-dimensional cell spheres (three-dimensional cell co-culture assay) that contain a large amount of glioma cells, mimicking the late stage of glioma growth, the phagocytosis of microglia was suppressed, which suggests that glioma cells could reeducate classically activated microglia into a tumor-promoting state at some point during tumor progression. Notably, we found that microglia could contribute to tumor invasion and acquisition of the epithelial-mesenchymal transition phenotype in the glioma microenvironment during the early stage and the late stage of tumor progression. In conclusion, we have developed a potential quantitative method for in vitro study of glioma immunity and provided evidence for the duality of glioma-associated microglia.
关键词[WOS]: ACTIVATED MICROGLIA ;  CELLS ;  TRANSITION ;  MATRIX ;  MECHANISMS ;  MACROPHAGE ;  RESISTANT ;  MIGRATION ;  INVASION ;  SYSTEM
语种: 英语
WOS记录号: WOS:000399906000021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151975
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, West Sect 9, Dept Physiol, Lvshun South Rd, Dalian 116044, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Med Univ, West Sect 9, Dept Immunol, Lvshun South Rd, Dalian 116044, Peoples R China
4.Gen Hosp Chengdu Army, Ctr Lab Dept, Chengdu 610083, Peoples R China
5.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China

Recommended Citation:
Gu, Rui,Zhang, Xu,Zhang, Ge,et al. Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice[J]. NEUROCHEMICAL RESEARCH,2017,42(5):1478-1487.
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