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题名: Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides
作者: Jiao, Fenglong1, 2;  Gao, Fangyuan2;  Wang, Heping2;  Deng, Yulin1;  Zhang, Yangjun2;  Qian, Xiaohong2;  Zhang, Yukui3
关键词: Au nanowires ;  Graphene oxide ;  L-Cys ;  Glycopeptides ;  Enrichment
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2017-06-01
DOI: 10.1016/j.aca.2017.03.014
卷: 970, 页:47-56
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Protein glycosylation has been proven to participate in a variety of complex biological processes; however, the low abundance of glycopeptides in natural samples makes it essential to develop methods to isolate and enrich glycopeptides. In this study, a novel ultrathin Au nanowire assisted zwitterionic hydrophilic magnetic graphene oxide ( GO-Fe3O4/SiO2/AuNWs/L-Cys) was synthesized with the good biocompatibility of GO, strong magnetic responses of Fe3O4, large surface area of ultrathin Au nanowires and excellent hydrophilicity of L-Cys via four simple and rapid steps. The ultrathin Au nanowires have a one-dimensional structure and were easily grafted with an abundant amount of L-Cys for the enrichment of glycopeptides. After the GO-Fe3O4/SiO2/AuNWs/L-Cys composites were applied to glycopeptide enrichment, 26 glycopeptides from a human IgG digest could be identified, with a detection limit as low as 10 fmol. Due to the abundant amount of grafted L-Cys, the composites also showed a large binding capacity ( 150 mu g mg(-1)). Furthermore, the composites were applied for the analysis of real biological samples. A total of 793 glycopeptides from 467 glycoproteins were identified in three replicate analyses of 40 mg of mouse liver proteins. The results demonstrated the great potential of GO-Fe3O4/SiO2/AuNWs/L-Cys composites for the analysis of glycoproteins. (C) 2017 Elsevier B.V. All rightd reserved.
关键词[WOS]: SELECTIVE ENRICHMENT ;  MASS-SPECTROMETRY ;  FACILE SYNTHESIS ;  OXIDE ;  NANOPARTICLES ;  GLYCOPROTEINS ;  EFFICIENT ;  GLYCOPROTEINS/GLYCOPEPTIDES ;  NANOCOMPOSITES ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000399781700005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151978
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
2.Natl Ctr Prot Sci, State Key Lab Prote, Beijing 102200, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Jiao, Fenglong,Gao, Fangyuan,Wang, Heping,et al. Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides[J]. ANALYTICA CHIMICA ACTA,2017,970:47-56.
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