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Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides
Jiao, Fenglong1,2; Gao, Fangyuan2; Wang, Heping2; Deng, Yulin1; Zhang, Yangjun2; Qian, Xiaohong2; Zhang, Yukui3
KeywordAu Nanowires Graphene Oxide L-cys Glycopeptides Enrichment
Source PublicationANALYTICA CHIMICA ACTA
2017-06-01
DOI10.1016/j.aca.2017.03.014
Volume970Pages:47-56
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSELECTIVE ENRICHMENT ; MASS-SPECTROMETRY ; FACILE SYNTHESIS ; OXIDE ; NANOPARTICLES ; GLYCOPROTEINS ; EFFICIENT ; GLYCOPROTEINS/GLYCOPEPTIDES ; NANOCOMPOSITES ; IDENTIFICATION
AbstractProtein glycosylation has been proven to participate in a variety of complex biological processes; however, the low abundance of glycopeptides in natural samples makes it essential to develop methods to isolate and enrich glycopeptides. In this study, a novel ultrathin Au nanowire assisted zwitterionic hydrophilic magnetic graphene oxide ( GO-Fe3O4/SiO2/AuNWs/L-Cys) was synthesized with the good biocompatibility of GO, strong magnetic responses of Fe3O4, large surface area of ultrathin Au nanowires and excellent hydrophilicity of L-Cys via four simple and rapid steps. The ultrathin Au nanowires have a one-dimensional structure and were easily grafted with an abundant amount of L-Cys for the enrichment of glycopeptides. After the GO-Fe3O4/SiO2/AuNWs/L-Cys composites were applied to glycopeptide enrichment, 26 glycopeptides from a human IgG digest could be identified, with a detection limit as low as 10 fmol. Due to the abundant amount of grafted L-Cys, the composites also showed a large binding capacity ( 150 mu g mg(-1)). Furthermore, the composites were applied for the analysis of real biological samples. A total of 793 glycopeptides from 467 glycoproteins were identified in three replicate analyses of 40 mg of mouse liver proteins. The results demonstrated the great potential of GO-Fe3O4/SiO2/AuNWs/L-Cys composites for the analysis of glycoproteins. (C) 2017 Elsevier B.V. All rightd reserved.
Language英语
WOS IDWOS:000399781700005
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/151978
Collection中国科学院大连化学物理研究所
Affiliation1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
2.Natl Ctr Prot Sci, State Key Lab Prote, Beijing 102200, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Jiao, Fenglong,Gao, Fangyuan,Wang, Heping,et al. Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides[J]. ANALYTICA CHIMICA ACTA,2017,970:47-56.
APA Jiao, Fenglong.,Gao, Fangyuan.,Wang, Heping.,Deng, Yulin.,Zhang, Yangjun.,...&Zhang, Yukui.(2017).Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides.ANALYTICA CHIMICA ACTA,970,47-56.
MLA Jiao, Fenglong,et al."Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides".ANALYTICA CHIMICA ACTA 970(2017):47-56.
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