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Detecting Proteins Glycosylation by a Homogeneous Reaction System with Zwitterionic Gold Nanoclusters
Li, Jinan1,3; Liu, Jing1,4; Liu, Zheyi1,3; Tan, Yuan2,3; Liu, Xiaoyan2; Wang, Fangjun1
刊名ANALYTICAL CHEMISTRY
2017-04-18
DOI10.1021/acs.analchem.6b04880
89期:8页:4339-4343
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HIGHLY EFFICIENT ENRICHMENT ; SELECTIVE ENRICHMENT ; MAGNETIC NANOPARTICLES ; DIRECTED SYNTHESIS ; MASS-SPECTROMETRY ; CRYSTAL-STRUCTURE ; GLYCOPEPTIDES ; CLUSTER ; CELLS ; SIZE
英文摘要Homogeneous gold nanoclusters (Au NCs) have been widely utilized in drug dejivery, chemical sensing, bioassays, and biolabeling due to their unique physical and chemical properties. However, little attention has been paid to their application in detecting protein post-translational modifications. Herein, we describe the development of a homogeneous reaction system with water-soluble zwitterionic Au NCs to capture glycopeptides from complex biological samples. The unique characteristics of Au NCs, such as their molecular-like properties, the excellent homogeneity in aqueous solution, the organic solvent responsive precipitation, and the easy preparation in only 4.5 h, contribute to the high efficiency and high throughput for capturing the targeted glycopeptides. Compared with the conventional heterogeneous system with solid-state adsorbents, the number of characterized glycosylation sites was improved by 35%. Finally, an MS detection limit as low as 50 amol was achieved for the standard glycoprotein (IgG), and 1576 glycosylation sites from 713 glycoproteins were identified from only 60 jig of mouse liver protein. Data are available via ProteomeXchange with identifier PXDO05635.
语种英语
WOS记录号WOS:000399858800003
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/151979
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
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GB/T 7714
Li, Jinan,Liu, Jing,Liu, Zheyi,et al. Detecting Proteins Glycosylation by a Homogeneous Reaction System with Zwitterionic Gold Nanoclusters[J]. ANALYTICAL CHEMISTRY,2017,89(8):4339-4343.
APA Li, Jinan,Liu, Jing,Liu, Zheyi,Tan, Yuan,Liu, Xiaoyan,&Wang, Fangjun.(2017).Detecting Proteins Glycosylation by a Homogeneous Reaction System with Zwitterionic Gold Nanoclusters.ANALYTICAL CHEMISTRY,89(8),4339-4343.
MLA Li, Jinan,et al."Detecting Proteins Glycosylation by a Homogeneous Reaction System with Zwitterionic Gold Nanoclusters".ANALYTICAL CHEMISTRY 89.8(2017):4339-4343.
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