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题名: Facile Preparation of Titanium(IV)-Immobilized Hierarchically Porous Hybrid Monoliths
作者: Zhang, Haiyang1, 2, 3;  Ou, Junjie1;  Yao, Yating1, 3;  Wang, Hongwei1, 3;  Liu, Zhongshan1, 3;  Wei, Yinmao2;  Ye, Mingliang1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-04-18
DOI: 10.1021/acs.analchem.7b00242
卷: 89, 期:8, 页:4655-4662
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Hierarchically porous materials have become a key feature of biological materials and have been widely applied for adsorption or catalysis. Herein, we presented a. new approach to directly prepare a phosphate-functionalized hierarchically porous hybrid monolith (HPHM), which simultaneously contained mesopores and macropores. The design was based on the copolymerization of polyhedral oligomeric vinylsilsesquioxanes (VinylPOSS) and vinyiphosphonic acid (VPA) by adding degradable polycaprolactone (PCL) additive. The phosphate groups could be directly introduced into the hybrid monoliths. This approach was Simple and time-saving, and overcame the defect of a rigorous, complex process for preparing traditional Ti4+-immobilized metal ion affinity chromatography (IMAC) materials. The specific surface area of an-optimal hybrid-monolith could reach 502 m(2)/g obtained by nitrogen adsorption/desorption measurements, which originated from the degradation of PCL. Meanwhile, the characterization of scanning electron microscopy (SEM) and mercury intrusion porosimetry (MIP) also suggested that the macropores existed in the hybrid monoliths. The size of macropores could be controlled by the content of PCL in the polymerization mixture. The prepared Ti4+-IMAC HPHMs exhibited high adsorption capacity (63.6 mg/g for pyridocal 5'-phosphatemonohydrate), and excellent enrichment specificity (tryptic digest of beta-casein/BSA at a molar ratio of 1:1000) and sensitivity (tryptic digest of 5 fmol of beta-casein). Moreover, the Ti4+-IMAC HPHMs provided effective enrichment ability of low-abundance phosphopeptides from human serum and HeLa cell digests.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  SELECTIVE ENRICHMENT ;  MASS-SPECTROMETRY ;  CAPILLARY COLUMN ;  PROTEIN-PHOSPHORYLATION ;  MICROPHASE SEPARATION ;  POLYMER MONOLITHS ;  BLOCK-COPOLYMERS ;  IN-VIVO ;  PHOSPHOPEPTIDES
语种: 英语
WOS记录号: WOS:000399858800050
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/151999
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Northwest Univ, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol Chem, Xian 710069, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Haiyang,Ou, Junjie,Yao, Yating,et al. Facile Preparation of Titanium(IV)-Immobilized Hierarchically Porous Hybrid Monoliths[J]. ANALYTICAL CHEMISTRY,2017,89(8):4655-4662.
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