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题名: Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation
作者: Liu, Hongdi1, 2;  Jiao, Xiang2;  Wang, Yanan2;  Yang, Xiaobing2, 3;  Sun, Wenyi2;  Wang, Jihui1;  Zhang, Sufang2, 3;  Zhao, Zongbao Kent2, 3
关键词: electroporation ;  linear DNA fragment ;  Rhodosporidium toruloides ;  genetic transformation ;  oleaginous yeast
刊名: FEMS YEAST RESEARCH
发表日期: 2017-03-01
DOI: 10.1093/femsyr/fox017
卷: 17, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
英文摘要: Metabolic engineering of Rhodosporidium toruloides, a robust lipid and caroteinoid producer, is of great importance for oleochemicals and carotenoids production. However, the Agrobacterium-mediated gene transformation is tedious and time consuming. Here, we described a fast and efficient genetic transformation of R. toruloides using electroporation with linear DNA fragments, and the process was optimized. The results showed that 2 x 10(3) transformants can be obtained at 0.7 kV/mu g linear DNA by using hygromycin and bleomycin as selection markers after the competent cells pretreated with 25 mM DTT and 100 mM LiAc. Our results would facilitate mutant library construction and metabolic engineering of R. toruloides for production of oleochemicals and carotenoids. We further demonstrated that all transformants arose due to illegitimate integration of transforming DNA fragments by colony PCR.
关键词[WOS]: CELL-WALL POROSITY ;  SACCHAROMYCES-CEREVISIAE ;  CRYPTOCOCCUS-NEOFORMANS ;  LIPID PRODUCTION ;  SCHIZOSACCHAROMYCES-POMBE ;  SELECTIVE MARKER ;  RHODOTORULA ;  PROMOTERS ;  DELETION ;  CLONING
语种: 英语
WOS记录号: WOS:000400577600007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152011
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China

Recommended Citation:
Liu, Hongdi,Jiao, Xiang,Wang, Yanan,et al. Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation[J]. FEMS YEAST RESEARCH,2017,17(2).
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