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Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation
Liu, Hongdi1,2; Jiao, Xiang2; Wang, Yanan2; Yang, Xiaobing2,3; Sun, Wenyi2; Wang, Jihui1; Zhang, Sufang2,3; Zhao, Zongbao Kent2,3
关键词Electroporation Linear Dna Fragment Rhodosporidium Toruloides Genetic Transformation Oleaginous Yeast
刊名FEMS YEAST RESEARCH
2017-03-01
DOI10.1093/femsyr/fox017
17期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Microbiology ; Mycology
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology ; Mycology
关键词[WOS]CELL-WALL POROSITY ; SACCHAROMYCES-CEREVISIAE ; CRYPTOCOCCUS-NEOFORMANS ; LIPID PRODUCTION ; SCHIZOSACCHAROMYCES-POMBE ; SELECTIVE MARKER ; RHODOTORULA ; PROMOTERS ; DELETION ; CLONING
英文摘要Metabolic engineering of Rhodosporidium toruloides, a robust lipid and caroteinoid producer, is of great importance for oleochemicals and carotenoids production. However, the Agrobacterium-mediated gene transformation is tedious and time consuming. Here, we described a fast and efficient genetic transformation of R. toruloides using electroporation with linear DNA fragments, and the process was optimized. The results showed that 2 x 10(3) transformants can be obtained at 0.7 kV/mu g linear DNA by using hygromycin and bleomycin as selection markers after the competent cells pretreated with 25 mM DTT and 100 mM LiAc. Our results would facilitate mutant library construction and metabolic engineering of R. toruloides for production of oleochemicals and carotenoids. We further demonstrated that all transformants arose due to illegitimate integration of transforming DNA fragments by colony PCR.
语种英语
WOS记录号WOS:000400577600007
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152011
专题中国科学院大连化学物理研究所
作者单位1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
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Liu, Hongdi,Jiao, Xiang,Wang, Yanan,et al. Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation[J]. FEMS YEAST RESEARCH,2017,17(2).
APA Liu, Hongdi.,Jiao, Xiang.,Wang, Yanan.,Yang, Xiaobing.,Sun, Wenyi.,...&Zhao, Zongbao Kent.(2017).Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation.FEMS YEAST RESEARCH,17(2).
MLA Liu, Hongdi,et al."Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation".FEMS YEAST RESEARCH 17.2(2017).
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