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Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation
Liu, Hongdi1,2; Jiao, Xiang2; Wang, Yanan2; Yang, Xiaobing2,3; Sun, Wenyi2; Wang, Jihui1; Zhang, Sufang2,3; Zhao, Zongbao Kent2,3
KeywordElectroporation Linear Dna Fragment Rhodosporidium Toruloides Genetic Transformation Oleaginous Yeast
Source PublicationFEMS YEAST RESEARCH
2017-03-01
DOI10.1093/femsyr/fox017
Volume17Issue:2
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS KeywordCELL-WALL POROSITY ; SACCHAROMYCES-CEREVISIAE ; CRYPTOCOCCUS-NEOFORMANS ; LIPID PRODUCTION ; SCHIZOSACCHAROMYCES-POMBE ; SELECTIVE MARKER ; RHODOTORULA ; PROMOTERS ; DELETION ; CLONING
AbstractMetabolic engineering of Rhodosporidium toruloides, a robust lipid and caroteinoid producer, is of great importance for oleochemicals and carotenoids production. However, the Agrobacterium-mediated gene transformation is tedious and time consuming. Here, we described a fast and efficient genetic transformation of R. toruloides using electroporation with linear DNA fragments, and the process was optimized. The results showed that 2 x 10(3) transformants can be obtained at 0.7 kV/mu g linear DNA by using hygromycin and bleomycin as selection markers after the competent cells pretreated with 25 mM DTT and 100 mM LiAc. Our results would facilitate mutant library construction and metabolic engineering of R. toruloides for production of oleochemicals and carotenoids. We further demonstrated that all transformants arose due to illegitimate integration of transforming DNA fragments by colony PCR.
Language英语
WOS IDWOS:000400577600007
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152011
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hongdi,Jiao, Xiang,Wang, Yanan,et al. Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation[J]. FEMS YEAST RESEARCH,2017,17(2).
APA Liu, Hongdi.,Jiao, Xiang.,Wang, Yanan.,Yang, Xiaobing.,Sun, Wenyi.,...&Zhao, Zongbao Kent.(2017).Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation.FEMS YEAST RESEARCH,17(2).
MLA Liu, Hongdi,et al."Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation".FEMS YEAST RESEARCH 17.2(2017).
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