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题名: Engineering islet for improved performance by optimized reaggregation in alginate gel beads
作者: Li, Na1, 2, 3;  Sun, Guangwei1;  Wang, Shujun2;  Wang, Yu1, 3;  Xiu, Zhilong2;  Sun, Dongsheng1, 2, 3;  Guo, Xin1;  Zhang, Ying1;  Ma, Xiaojun1
关键词: encapsulation ;  engineering islets ;  islets isolation ;  islet cell clusters ;  reaggregation
刊名: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
发表日期: 2017-05-01
DOI: 10.1002/bab.1489
卷: 64, 期:3, 页:400-405
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: After islet isolation, diffusion has become the main mechanism to transport oxygen and nutrients into the core of islets. However, diffusion has limitations, by which nutrients cannot effectively reach the core of large islets and can eventually cause core cell death and islet loss. This problem can be resolved by dispersing islets into single islet cells, but single islet cells do not exhibit insulin release function in in vitro culture. In this study, we intended to establish a new islet engineering approach by forming islet cell clusters to improve islet survival and function. Therefore, alginate gels were used to encapsulate islet cells to form artificial islets after dispersion of islets into single cells. The shape of the islet cell clusters was similar to native islets, and the size of the islet cell clusters was limited to a maximum diameter of 100 mu m. By limiting the diameter of this engineered islet cell cluster, cell viability was nearly 100%, a significant improvement over natural islets. Importantly, islet cell clusters express the genes of islets, including Isl-1, Gcg, and insulin-1, and insulin secretion ability was maintained in vitro. C (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.
关键词[WOS]: INSULIN-SECRETION ;  BETA-CELL ;  TRANSPLANTATION ;  SURVIVAL ;  RELEASE
语种: 英语
WOS记录号: WOS:000405275100010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152026
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China

Recommended Citation:
Li, Na,Sun, Guangwei,Wang, Shujun,et al. Engineering islet for improved performance by optimized reaggregation in alginate gel beads[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2017,64(3):400-405.
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