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Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer
Mayang, Yanchao1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3
KeywordSurface Plasmon Resonance Self-assembled Monolayer Detection Of Glycoproteins Transferrin. Boronate Affinity Materials
Source PublicationMICROCHIMICA ACTA
2017-08-01
DOI10.1007/s00604-017-2275-3
Volume184Issue:8Pages:2749-2757
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTRANSFER RADICAL POLYMERIZATION ; CLICK CHEMISTRY ; IRON TRANSPORT ; LABEL-FREE ; GLYCOPROTEINS ; BIOSENSOR ; NANOPARTICLES ; ENRICHMENT ; PROTEINS ; RECOGNITION
AbstractA surface plasmon resonance sensor whose surface has been modified with boronic acid groups was developed in order to bind and detect transferrin. 4-Mercaptophenylboronic acid (4-MPBA) was utilized to form a self-assembled monolayer on the gold surface of the sensor chip. The surface topography of the modified gold chip was characterized by atomic force microscopy. In solutions of pH 9, the glycosyl groups of transferrin (Trf) quickly and reversibly combine with 4-MPBA on the sensor chip to result in changes of the refractive index. Thus, Trf can be sensed online by monitoring the SPR signal. Under optimized conditions, the sensor has a linear response in the 10 nM to 1.3 mu M Trf concentration range, and the limit of detection is 4.4 nM (equivalent to 0.34 mu g.mL(-1)). The results suggest that this 4-MPBA-functionalized sensor is a viable candidate for clinical testing of Trf which is a potential marker for severe diseases such as hypertension, primary renal disease, and diabetes.
Language英语
WOS IDWOS:000406066500029
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152042
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Mayang, Yanchao,He, Xiwen,Chen, Langxing,et al. Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer[J]. MICROCHIMICA ACTA,2017,184(8):2749-2757.
APA Mayang, Yanchao,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer.MICROCHIMICA ACTA,184(8),2749-2757.
MLA Mayang, Yanchao,et al."Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer".MICROCHIMICA ACTA 184.8(2017):2749-2757.
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