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题名: Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer
作者: Mayang, Yanchao1, 2;  He, Xiwen1, 2;  Chen, Langxing1, 2;  Zhang, Yukui1, 2, 3
关键词: Surface plasmon resonance ;  Self-assembled monolayer ;  Detection of glycoproteins ;  Transferrin. ;  Boronate affinity materials
刊名: MICROCHIMICA ACTA
发表日期: 2017-08-01
DOI: 10.1007/s00604-017-2275-3
卷: 184, 期:8, 页:2749-2757
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A surface plasmon resonance sensor whose surface has been modified with boronic acid groups was developed in order to bind and detect transferrin. 4-Mercaptophenylboronic acid (4-MPBA) was utilized to form a self-assembled monolayer on the gold surface of the sensor chip. The surface topography of the modified gold chip was characterized by atomic force microscopy. In solutions of pH 9, the glycosyl groups of transferrin (Trf) quickly and reversibly combine with 4-MPBA on the sensor chip to result in changes of the refractive index. Thus, Trf can be sensed online by monitoring the SPR signal. Under optimized conditions, the sensor has a linear response in the 10 nM to 1.3 mu M Trf concentration range, and the limit of detection is 4.4 nM (equivalent to 0.34 mu g.mL(-1)). The results suggest that this 4-MPBA-functionalized sensor is a viable candidate for clinical testing of Trf which is a potential marker for severe diseases such as hypertension, primary renal disease, and diabetes.
关键词[WOS]: TRANSFER RADICAL POLYMERIZATION ;  CLICK CHEMISTRY ;  IRON TRANSPORT ;  LABEL-FREE ;  GLYCOPROTEINS ;  BIOSENSOR ;  NANOPARTICLES ;  ENRICHMENT ;  PROTEINS ;  RECOGNITION
语种: 英语
WOS记录号: WOS:000406066500029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152042
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Mayang, Yanchao,He, Xiwen,Chen, Langxing,et al. Detection of transferrin by using a surface plasmon resonance sensor functionalized with a boronic acid monolayer[J]. MICROCHIMICA ACTA,2017,184(8):2749-2757.
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