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题名: Evaluation of red and near infrared fluorescent silver nanoclusters as potential in vivo indicators of tight junction opening
作者: Wang, Xinyi1;  Wang, Na3;  Li, Limei1;  Xiao, Ruyue2;  Yuan, Lan2;  Yang, Xiaoda2;  Li, Na4
刊名: RSC ADVANCES
发表日期: 2017
DOI: 10.1039/c7ra05561g
卷: 7, 页:32536-32542
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Tight junctions (TJs) play a key role in regulating permeability to liquids, ions and larger solutes through the paracellular route. To demonstrate TJ structure changes by measuring paracellular flux is challenging for understanding biological functions of tight junction and designing delivery system for highly hydrophilic macromolecular drugs. In the present study, we tested two long wavelength emitting silver nanoclusters (AgNCs), Ag2S(BSA)-NCs (lambda(ex/em) at 500/1050 nm) and Ag(GSH)-NCs (lambda(ex/em) at 488/640 nm), for their suitability as novel paracellular permeation indicators on a MDCK monolayer. Ag2S(BSA)-NCs exhibited marginal cytotoxicity and passed through the cell monolayer exclusively via the paracellular pathway. However, Ag(GSH)-NCs could be taken inside the cells possibly through endocytosis. AgNCs together with Eu-DTPA were used in the double probe strategy for detecting the change of TJ pore path parameters (i.e. pore size r and retention capacity epsilon/tau) upon TJ opening with EDTA or vanadyl acetylacetonates, respectively. The AgNCs/Eu-DTPA probe set was found to give the same results as our previous work using the short wavelength emitting AuNCs and Eu-DTPA probe set, suggesting future potential applications of AgNCs to the in vivo studies of TJ alternations upon stresses. This work reinforced the use of a double probe set for study of the TJ structure change.
关键词[WOS]: BLOOD-BRAIN-BARRIER ;  OPTICAL-PROPERTIES ;  PERMEABILITY ;  CELLS ;  DELIVERY ;  CHITOSAN ;  CACO-2 ;  PERMEATION ;  MODULATION ;  MONOLAYERS
语种: 英语
WOS记录号: WOS:000404609800012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152065
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Hebei Med Univ, Sch Pharmaceut Sci, Dept Pharmacognosy, Shijiazhuang 050017, Peoples R China
4.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Xinyi,Wang, Na,Li, Limei,et al. Evaluation of red and near infrared fluorescent silver nanoclusters as potential in vivo indicators of tight junction opening[J]. RSC ADVANCES,2017,7:32536-32542.
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