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Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae
Tian, Lirong1,2; Liu, Zheyi3; Wang, Fangjun3; Shen, Liangliang1,2; Chen, Jinghua1,2; Chang, Lijing1,2; Zhao, Songhao1,2; Han, Guangye1; Wang, Wenda1; Kuang, Tingyun1; Qin, Xiaochun1,4; Shen, Jian-Ren1,5
KeywordCyanidioschyzon Merolae Psi-lhci Light-harvesting Zeaxanthin Red Algae
Source PublicationPHOTOSYNTHESIS RESEARCH
2017-09-01
DOI10.1007/s11120-017-0384-9
Volume133Pages:201-214
Indexed BySCI ; ISTP
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectPlant Sciences
WOS Research AreaPlant Sciences
WOS KeywordPLANT PHOTOSYSTEM-I ; LIGHT-HARVESTING COMPLEXES ; ENERGY-TRANSFER PATHWAYS ; CHLAMYDOMONAS-REINHARDTII ; GALDIERIA-SULPHURARIA ; PORPHYRIDIUM-CRUENTUM ; CYANIDIUM-CALDARIUM ; GREEN-ALGA ; SUBUNIT ; SUPERCOMPLEX
AbstractPhotosystem I (PSI)-light-harvesting complex I (LHCI) super-complex and its sub-complexes PSI core and LHCI, were purified from a unicellular red alga Cyanidioschyzon merolae and characterized. PSI-LHCI of C. merolae existed as a monomer with a molecular mass of 580 kDa. Mass spectrometry analysis identified 11 subunits (PsaA, B, C, D, E, F, I, J, K, L, O) in the core complex and three LHCI subunits, CMQ142C, CMN234C, and CMN235C in LHCI, indicating that at least three Lhcr subunits associate with the red algal PSI core. PsaG was not found in the red algae PSI-LHCI, and we suggest that the position corresponding to Lhca1 in higher plant PSI-LHCI is empty in the red algal PSI-LHCI. The PSI-LHCI complex was separated into two bands on native PAGE, suggesting that two different complexes may be present with slightly different protein compositions probably with respective to the numbers of Lhcr subunits. Based on the results obtained, a structural model was proposed for the red algal PSI-LHCI. Furthermore, pigment analysis revealed that the C. merolae PSI-LHCI contained a large amount of zeaxanthin, which is mainly associated with the LHCI complex whereas little zeaxanthin was found in the PSI core. This indicates a unique feature of the carotenoid composition of the Lhcr proteins and may suggest an important role of Zea in the light-harvesting and photoprotection of the red algal PSI-LHCI complex.
Language英语
WOS IDWOS:000405086400018
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152069
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Zhongshan Rd 457, Dalian 116023, Peoples R China
4.Univ Jinan, Sch Biol Sci & Technol, 336 Nanxinzhuang West Rd, Jinan 250022, Peoples R China
5.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan
Recommended Citation
GB/T 7714
Tian, Lirong,Liu, Zheyi,Wang, Fangjun,et al. Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae[J]. PHOTOSYNTHESIS RESEARCH,2017,133:201-214.
APA Tian, Lirong.,Liu, Zheyi.,Wang, Fangjun.,Shen, Liangliang.,Chen, Jinghua.,...&Shen, Jian-Ren.(2017).Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae.PHOTOSYNTHESIS RESEARCH,133,201-214.
MLA Tian, Lirong,et al."Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae".PHOTOSYNTHESIS RESEARCH 133(2017):201-214.
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