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Relations between the loop transposition of DNA G-quadruplex and the catalytic function of DNAzyme
Cheng, Mingpan1,2; Zhou, Jun3; Jia, Guoqing1; Ai, Xuanjun4; Mergny, Jean-Louis5,6; Li, Can1,4
KeywordG-quadruplex Loop Transposition Dnazyme Dna Catalysis Hemin G-quadruplex Ligand
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
2017-08-01
DOI10.1016/j.bbagen.2017.05.016
Volume1861Pages:1913-1920
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS KeywordTELOMERIC G-QUADRUPLEX ; PEROXIDASE-MIMICKING DNAZYME ; INTRAMOLECULAR G-QUADRUPLEX ; G-RICH OLIGONUCLEOTIDE ; K+ SOLUTION ; CIRCULAR-DICHROISM ; ACTIVITY ENHANCEMENT ; POTASSIUM SOLUTION ; THYMINE DIMERS ; NUCLEIC-ACIDS
AbstractThe structures of DNA G-quadruplexes are essential for their functions in vivo and in vitro. Our present study revealed that sequential order of the three G-quadruplex loops, that is, loop transposition, could be a critical factor to determinate the G-quadruplex conformation and consequently improved the catalytic function of G-quadruplex based DNAzyme. In the presence, of 100 mM K+, loop transposition induced one of the G-quadruplex isomers which shared identical loops but differed in the sequential order of loops into a hybrid topology while the others into predominately parallel topologies. D-1 NMR spectroscopy and mutation analysis suggested that the hydrogen bonding from loops residues with nucleotides in flanking sequences may be responsible for the stabilization of the different conformations. A well-known DNAzyme consisting of G-quadruplex and hemin (Ferriprotoporphyrin IX chloride) was chosen to test the catalytic function. We found that the loop transposition could enhance the reaction rate obviously by increasing the hemin binding affinity to G-quadruplex. These findings disclose the relations between the loop transposition, G-quadruplex conformation and catalytic function of DNAzyme.
Language英语
WOS IDWOS:000404702600003
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152075
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
3.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
5.Univ Bordeaux, IECB, ARNA Lab, CNRS,UMR5320,Inserm U1212, Pessac, France
6.Inst Biophys CAS, Vvi, Kralovopolska 135, Brno 61265, Czech Republic
Recommended Citation
GB/T 7714
Cheng, Mingpan,Zhou, Jun,Jia, Guoqing,et al. Relations between the loop transposition of DNA G-quadruplex and the catalytic function of DNAzyme[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2017,1861:1913-1920.
APA Cheng, Mingpan,Zhou, Jun,Jia, Guoqing,Ai, Xuanjun,Mergny, Jean-Louis,&Li, Can.(2017).Relations between the loop transposition of DNA G-quadruplex and the catalytic function of DNAzyme.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1861,1913-1920.
MLA Cheng, Mingpan,et al."Relations between the loop transposition of DNA G-quadruplex and the catalytic function of DNAzyme".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1861(2017):1913-1920.
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