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题名: Relations between the loop transposition of DNA G-quadruplex and the catalytic function of DNAzyme
作者: Cheng, Mingpan1, 2;  Zhou, Jun3;  Jia, Guoqing1;  Ai, Xuanjun4;  Mergny, Jean-Louis5, 6;  Li, Can1, 4
关键词: G-quadruplex ;  Loop transposition ;  DNAzyme ;  DNA catalysis ;  Hemin ;  G-quadruplex ligand
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
发表日期: 2017-08-01
DOI: 10.1016/j.bbagen.2017.05.016
卷: 1861, 页:1913-1920
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: The structures of DNA G-quadruplexes are essential for their functions in vivo and in vitro. Our present study revealed that sequential order of the three G-quadruplex loops, that is, loop transposition, could be a critical factor to determinate the G-quadruplex conformation and consequently improved the catalytic function of G-quadruplex based DNAzyme. In the presence, of 100 mM K+, loop transposition induced one of the G-quadruplex isomers which shared identical loops but differed in the sequential order of loops into a hybrid topology while the others into predominately parallel topologies. D-1 NMR spectroscopy and mutation analysis suggested that the hydrogen bonding from loops residues with nucleotides in flanking sequences may be responsible for the stabilization of the different conformations. A well-known DNAzyme consisting of G-quadruplex and hemin (Ferriprotoporphyrin IX chloride) was chosen to test the catalytic function. We found that the loop transposition could enhance the reaction rate obviously by increasing the hemin binding affinity to G-quadruplex. These findings disclose the relations between the loop transposition, G-quadruplex conformation and catalytic function of DNAzyme.
关键词[WOS]: TELOMERIC G-QUADRUPLEX ;  PEROXIDASE-MIMICKING DNAZYME ;  INTRAMOLECULAR G-QUADRUPLEX ;  G-RICH OLIGONUCLEOTIDE ;  K+ SOLUTION ;  CIRCULAR-DICHROISM ;  ACTIVITY ENHANCEMENT ;  POTASSIUM SOLUTION ;  THYMINE DIMERS ;  NUCLEIC-ACIDS
语种: 英语
WOS记录号: WOS:000404702600003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152075
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
3.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
5.Univ Bordeaux, IECB, ARNA Lab, CNRS,UMR5320,Inserm U1212, Pessac, France
6.Inst Biophys CAS, Vvi, Kralovopolska 135, Brno 61265, Czech Republic

Recommended Citation:
Cheng, Mingpan,Zhou, Jun,Jia, Guoqing,et al. Relations between the loop transposition of DNA G-quadruplex and the catalytic function of DNAzyme[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2017,1861:1913-1920.
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