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Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica
Meng, Yingying1,2; Cao, Xupeng2; Yao, Changhong2; Xue, Song2; Yang, Qing1
KeywordNannochloropsis Lipidomics Polar Glycerolipids Metabolism Tag Accumulation Nitrogen Starvation
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
2017-06-01
DOI10.1016/j.algal.2017.03.004
Volume24Pages:122-129
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordCHROMATOGRAPHY-MASS SPECTROMETRY ; DIATOM FISTULIFERA-SOLARIS ; CHLAMYDOMONAS-REINHARDTII ; EICOSAPENTAENOIC ACID ; TRIACYLGLYCEROL ACCUMULATION ; PHAEODACTYLUM-TRICORNUTUM ; LIQUID-CHROMATOGRAPHY ; NITROGEN DEPRIVATION ; FATTY-ACIDS ; BIOSYNTHESIS
AbstractNannochloropsis, an ocean microalgal genus, is a promising resource of biodiesel feedstock because of its capacity for triacylglycerol (TAG) accumulation. Polar glycerolipids have big contribution for TAG accumulation by providing acyl group or diacylglycerol (DAG). Due to the diversities and amount of polar glycerolipids in species, a suitable approach has not been developed well to quantify and qualify, which veils the clarification of how important roles the polar glycerolipids play in microalgal lipid metabolism and TAG accumulation. In this study, a total of 112 polar glycerolipid species, covering eight classes, were identified using UPLC/Q-TRAP plus Orbitrap coupled with quantified using UPLC/Q-TOF. A comprehensive profiling of polar glycerolipid was established to study the polar glycerolipids variations in response to nitrogen deprivation in Nannochloropsis oceanica. Through targeted lipidomics analysis it was proposed that in N. oceanica, phosphatidylcholine (PC) serve as linoleic and gamma-linolenic acyl donors via oleic acyl desaturation, and under nitrogen stress condition PC transfers acyl 16:0 and 18:1 to TAG. The result disclosed PC played import roles for TAG accumulation not only in high plants but also in PC-containing microalgae even PC is not the most extraplastidial lipid. In addition, it was showed that phosphatidylethanolamine (PE) and diacylglyceryl-N-trimethylhomoserine (DGTS) are suppliers of eicosapentaenoic acid (EPA) to other lipids. These new discoveries reveal the diversity of microalgae in acyl metabolism and shed light on the understanding of lipid biosynthesis by stoichiometry. (C) 2017 Published by Elsevier B.V.
Language英语
WOS IDWOS:000404864600012
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152077
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Meng, Yingying,Cao, Xupeng,Yao, Changhong,et al. Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,24:122-129.
APA Meng, Yingying,Cao, Xupeng,Yao, Changhong,Xue, Song,&Yang, Qing.(2017).Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,24,122-129.
MLA Meng, Yingying,et al."Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 24(2017):122-129.
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