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A fluorescent sensing for glycoproteins based on the FRET between quantum dots and Au nanoparticles
Chang, Lifang1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
关键词Mn-doped Zns Quantum Dots Au Nanoparticies Fluorescence Resonance Energy Transfer Glycoproteins
刊名SENSORS AND ACTUATORS B-CHEMICAL
2017-10-01
DOI10.1016/j.snb.2017.04.153
250页:17-23
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]RESONANCE ENERGY-TRANSFER ; TANDEM MASS-SPECTROMETRY ; GOLD NANOPARTICLES ; ALPHA(1)-ACID GLYCOPROTEIN ; SELECTIVE ENRICHMENT ; GLYCOFORM SEPARATION ; TURN-ON ; ACID ; STRATEGY ; ELECTROPHORESIS
英文摘要A simple fluorescent probe based on fluorescence resonance energy transfer (FRET) between the glucosamine-Mn-doped ZnS QDs and mercaptophenylboronic acid (MBA)-capped AuNPs was designed for the determination of glycoproteins such as immunoglobulin G (IgG), transferrin (Trf), alpha 1-acid glycoprotein (AGP) and horseradish peroxidase (HRP). The FRET process took place via interaction between the glucosamine on the surface of QDs and boronic acid moieties on AuNPs surface, and resulted in the fluorescence quenched of the Ws. The FRET efficiency from QDs to AuNPs was calculated to be 75.4%. However, the FRET process would be inhibited after glycoproteins were introduced into the QDs-AuNPs system. The stronger interaction between the MBA on the surface of AuNPs and glycoproteins would keep the AuNPs far away from the QDs surface, leading to the fluorescence recovered of the QDs. The as-prepared sensor showed a high sensitivity and selectivity for glycoproteins. The dissociation constants and the detection limits of the selected standard glycoproteins (Trf, HRP, IgG and AGP) were estimated to be around 10(-7) M and 10(-9) M, respectively. Finally, this sensor has been successfully used for the determination of the AGP in serum samples without any_ complicated pretreatment and the recovery was in the range of 70%-105%. (C) 2017 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000404424100003
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152095
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Chang, Lifang,He, Xiwen,Chen, Langxing,et al. A fluorescent sensing for glycoproteins based on the FRET between quantum dots and Au nanoparticles[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,250:17-23.
APA Chang, Lifang,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).A fluorescent sensing for glycoproteins based on the FRET between quantum dots and Au nanoparticles.SENSORS AND ACTUATORS B-CHEMICAL,250,17-23.
MLA Chang, Lifang,et al."A fluorescent sensing for glycoproteins based on the FRET between quantum dots and Au nanoparticles".SENSORS AND ACTUATORS B-CHEMICAL 250(2017):17-23.
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