中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification
作者: Yuan, Huiming1;  Zhang, Shen1, 2;  Zhao, Baofeng1;  Weng, Yejing1, 2;  Zhu, Xudong1, 2;  Li, Senwu1, 2;  Zhang, Lihua1;  Zhang, Yukui1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-06-20
DOI: 10.1021/acs.analchem.7b00682
卷: 89, 页:6324-6329
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Protein digestion and isotope, labeling are two critical steps in proteome quantification. However, the conventional in-solution protocol unavoidably suffers from disadvantages such as time-consuming, low labeling efficiency, and tedious off-line manual operation, which might affect the quantification accuracy, reproducibility, and throughput. To address these problems, we developed a fully automated proteome quantification platform, in which an ultraperformance immobilized microreactor (upIMER) with graphene-oxide-modified polymer micro spheres as the matrix was developed, to achieve not only the simultaneous protein digestion and O-18 labeling, but also the online integration, with nano-high-pressure liquid chromatography electrospray ionization-tandem mass spectrometry (nanoHPLC ESI-MS/MS). Compared to the conventional off-line protocols, such a platform exhibits obviously improved digestion and O-18 labeling efficiency (only 8% peptides with missed cleavage sites, 99% labeling efficiency, and 2.5 min reaction time), leading to the increased quantification coverage, accuracy, precision and throughput. All the results demonstrated that our developed fully automated platform should provide new opportunities to improve the accuracy, reproducibility, and throughput for proteome quantification.
关键词[WOS]: HIGHLY EFFICIENT ;  QUANTITATIVE PROTEOMICS ;  MASS-SPECTROMETRY ;  DIGESTION ;  METHODOLOGY ;  COMBINATION ;  STRATEGIES ;  PROTEINS ;  MIXTURES ;  CANCER
语种: 英语
WOS记录号: WOS:000404087600009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152111
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Yuan, Huiming,Zhang, Shen,Zhao, Baofeng,et al. Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification[J]. ANALYTICAL CHEMISTRY,2017,89:6324-6329.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Yuan, Huiming]'s Articles
 [Zhang, Shen]'s Articles
 [Zhao, Baofeng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Yuan, Huiming]‘s Articles
 [Zhang, Shen]‘s Articles
 [Zhao, Baofeng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace