DICP OpenIR
Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification
Yuan, Huiming1; Zhang, Shen1,2; Zhao, Baofeng1; Weng, Yejing1,2; Zhu, Xudong1,2; Li, Senwu1,2; Zhang, Lihua1; Zhang, Yukui1
Source PublicationANALYTICAL CHEMISTRY
2017-06-20
DOI10.1021/acs.analchem.7b00682
Volume89Pages:6324-6329
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHIGHLY EFFICIENT ; QUANTITATIVE PROTEOMICS ; MASS-SPECTROMETRY ; DIGESTION ; METHODOLOGY ; COMBINATION ; STRATEGIES ; PROTEINS ; MIXTURES ; CANCER
AbstractProtein digestion and isotope, labeling are two critical steps in proteome quantification. However, the conventional in-solution protocol unavoidably suffers from disadvantages such as time-consuming, low labeling efficiency, and tedious off-line manual operation, which might affect the quantification accuracy, reproducibility, and throughput. To address these problems, we developed a fully automated proteome quantification platform, in which an ultraperformance immobilized microreactor (upIMER) with graphene-oxide-modified polymer micro spheres as the matrix was developed, to achieve not only the simultaneous protein digestion and O-18 labeling, but also the online integration, with nano-high-pressure liquid chromatography electrospray ionization-tandem mass spectrometry (nanoHPLC ESI-MS/MS). Compared to the conventional off-line protocols, such a platform exhibits obviously improved digestion and O-18 labeling efficiency (only 8% peptides with missed cleavage sites, 99% labeling efficiency, and 2.5 min reaction time), leading to the increased quantification coverage, accuracy, precision and throughput. All the results demonstrated that our developed fully automated platform should provide new opportunities to improve the accuracy, reproducibility, and throughput for proteome quantification.
Language英语
WOS IDWOS:000404087600009
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152111
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Huiming,Zhang, Shen,Zhao, Baofeng,et al. Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification[J]. ANALYTICAL CHEMISTRY,2017,89:6324-6329.
APA Yuan, Huiming.,Zhang, Shen.,Zhao, Baofeng.,Weng, Yejing.,Zhu, Xudong.,...&Zhang, Yukui.(2017).Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification.ANALYTICAL CHEMISTRY,89,6324-6329.
MLA Yuan, Huiming,et al."Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification".ANALYTICAL CHEMISTRY 89(2017):6324-6329.
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