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题名: Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry
作者: Li, Wenxue1;  Petruzziello, Filomena1;  Zhao, Nan2;  Zhao, Huiyuan2;  Ye, Xueting3;  Zhang, Xiaozhe2;  Rainer, Gregor1
关键词: High-resolution mass spectrometry ;  Microproteins ;  Mouse brain ;  Peptides ;  Top down
刊名: PROTEOMICS
发表日期: 2017-06-17
DOI: 10.1002/pmic.201600419
卷: 17
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Microproteins and endogenous peptides in the brain contain important substances that have critical roles in diverse biological processes, contributing to signal transduction and intercellular signaling. However, variability in their physical or chemical characteristics, such as molecule size, hydrophobicity, and charge states, complicate the simultaneous analysis of these compounds, although this would be highly beneficial for the field of neuroscience research. Here, we present a top-down analytical method for simultaneous analysis of microproteins and endogenous peptides using high-resolution nanocapillary LC-MS/MS. This method is detergent-free and digestion-free, which allows for extracting and preserving intact microproteins and peptides for direct LC-MS analysis. Both higher energy collision dissociation and electron-transfer dissociation fragmentations were used in the LC-MS analysis to increase the identification rate, and bioinformatics tools ProteinGoggle and PEAKS Studio software were utilized for database search. In total, we identified 471 microproteins containing 736 proteoforms, including brain-derived neurotrophic factor and a number of fibroblast growth factors. In addition, we identified 599 peptides containing 151 known or potential neuropeptides such as somatostatin-28 and neuropeptide Y. Our approach bridges the gap for the characterization of brain microproteins and peptides, which permits quantification of a diversity of signaling molecules for biomarker discovery or therapy diagnosis in the future.
关键词[WOS]: NEUROPEPTIDES ;  PROTEOMICS ;  PROTEIN ;  AMIDATION ;  DISCOVERY ;  BDNF
语种: 英语
WOS记录号: WOS:000403899900006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152112
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Fribourg, Visual Cognit Lab, Dept Med, Fribourg, Switzerland
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Shenyang Pharmaceut Univ, Shenyang, Peoples R China

Recommended Citation:
Li, Wenxue,Petruzziello, Filomena,Zhao, Nan,et al. Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry[J]. PROTEOMICS,2017,17.
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