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Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry
Li, Wenxue1; Petruzziello, Filomena1; Zhao, Nan2; Zhao, Huiyuan2; Ye, Xueting3; Zhang, Xiaozhe2; Rainer, Gregor1
关键词High-resolution Mass Spectrometry Microproteins Mouse Brain Peptides Top Down
刊名PROTEOMICS
2017-06-17
DOI10.1002/pmic.201600419
17
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]NEUROPEPTIDES ; PROTEOMICS ; PROTEIN ; AMIDATION ; DISCOVERY ; BDNF
英文摘要Microproteins and endogenous peptides in the brain contain important substances that have critical roles in diverse biological processes, contributing to signal transduction and intercellular signaling. However, variability in their physical or chemical characteristics, such as molecule size, hydrophobicity, and charge states, complicate the simultaneous analysis of these compounds, although this would be highly beneficial for the field of neuroscience research. Here, we present a top-down analytical method for simultaneous analysis of microproteins and endogenous peptides using high-resolution nanocapillary LC-MS/MS. This method is detergent-free and digestion-free, which allows for extracting and preserving intact microproteins and peptides for direct LC-MS analysis. Both higher energy collision dissociation and electron-transfer dissociation fragmentations were used in the LC-MS analysis to increase the identification rate, and bioinformatics tools ProteinGoggle and PEAKS Studio software were utilized for database search. In total, we identified 471 microproteins containing 736 proteoforms, including brain-derived neurotrophic factor and a number of fibroblast growth factors. In addition, we identified 599 peptides containing 151 known or potential neuropeptides such as somatostatin-28 and neuropeptide Y. Our approach bridges the gap for the characterization of brain microproteins and peptides, which permits quantification of a diversity of signaling molecules for biomarker discovery or therapy diagnosis in the future.
语种英语
WOS记录号WOS:000403899900006
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152112
专题中国科学院大连化学物理研究所
作者单位1.Univ Fribourg, Visual Cognit Lab, Dept Med, Fribourg, Switzerland
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Shenyang Pharmaceut Univ, Shenyang, Peoples R China
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GB/T 7714
Li, Wenxue,Petruzziello, Filomena,Zhao, Nan,et al. Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry[J]. PROTEOMICS,2017,17.
APA Li, Wenxue.,Petruzziello, Filomena.,Zhao, Nan.,Zhao, Huiyuan.,Ye, Xueting.,...&Rainer, Gregor.(2017).Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry.PROTEOMICS,17.
MLA Li, Wenxue,et al."Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry".PROTEOMICS 17(2017).
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