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题名: Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice
作者: Gao Zhigang1;  Zheng Tingting1;  Deng Jiu1;  Li Xiaorui1;  Qu Yueyang1;  Lu Yao2;  Liu Tingjiao3;  Luo Yong1;  Zhao Weijie1;  Lin Bingcheng1
关键词: hair cortisol ;  SERS ;  paper microdevice ;  portable Raman spectrometer ;  competitive immunoassay
刊名: ACTA CHIMICA SINICA
发表日期: 2017-04-15
DOI: 10.6023/A16110598
卷: 75, 页:355-359
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Cortisol in the human hair is a clinical biomarker of long-term social-and-work-related mental stress, which is of high morbidity rate in the current modern society. This study developed a sensitive hair cortisol assay, featuring surface- enhanced Raman spectroscopy, immunoreaction, and a double-layer paper microdevice. The first layer of the paper microdevice was used to remove the hair residue in the hair extract by filtration (sample pretreatment). The second layer was used for competitive immunoreaction and detection. Standard cortisol antigen immobilized in the second layer and the free cortisol in hair extract competed to bind the spiked Raman-active cortisol monoclonal antibody solution. The hair cortisol can be quantitated by the intensity of Raman signal of monoclonal antibody bound on the paper. We found the Raman signal decreased as the cortisol concentration increases in hair samples. The relative Raman intensity measured was linearly proportional to the logarithmic value of the cortisol concentration in hair samples we measured. The detection of limit (LOD) was 1 pg/mL with the portable Raman spectrometer. The RSD of measurement was 8.38% (n=6). In addition, we used LC-MS to measure two real samples as a comparison with our method as above. The results are 0.771 and 0.153 ng/mL by LC-MS method and 0.63 and 0.247 ng/mL by the proposed method. It can be observed that the results are in same order, demonstrating the validity of the proposed method. In addition, 48 samples can be measured in a single chip. These results showed that this method is sensitive, specific, and suitable for large-scale screening of hair cortisol samples.
关键词[WOS]: MICROFLUIDIC PAPER ;  LOW-COST ;  SPECTROSCOPY ;  DEVICE
语种: 英语
WOS记录号: WOS:000402486800003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152197
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China
2.Dalian Inst Chem Phys, Biol Div, Dalian 116023, Peoples R China
3.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China

Recommended Citation:
Gao Zhigang,Zheng Tingting,Deng Jiu,et al. Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice[J]. ACTA CHIMICA SINICA,2017,75:355-359.
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