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"Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract
Sun, Guangying1,3; Liu, Yanfang2; Ahat, Hasanjan1,3; Shen, Aijin2; Liang, Xinmiao2; Xue, Xingya2; Luo, Yuqin1,3; Yang, Pan1,3; Liu, Zhao-Sheng1,3; Aisa, Haji Akber1,3
关键词"two-dimensional" Molecularly Imprinted Solid-phase Extraction Functional Complementation Crystallization High-performance Liquid Chromatography Pomegranate Husk Extract
刊名JOURNAL OF CHROMATOGRAPHY A
2017-07-07
DOI10.1016/j.chroma.2017.05.033
1505页:35-42
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ANTIOXIDANT ACTIVITIES ; FUNCTIONAL MONOMER ; WATER SAMPLES ; ELLAGIC ACID ; POLYMERS ; RECOGNITION ; PRODUCTS ; NANOPARTICLES ; TEMPLATES ; AFFINITY
英文摘要In this study, "two dimensional" molecularly imprinted solid-phase extraction (2D-MIP-SPE) of semi preparative grade was constructed to fast purify ellagitannins in pomegranate husk extract with the help of crystallization and reverse-phase liquid chromatgoraphy(RPLC). Ellagic acid and punicalagin imprinted polymers were synthesized in batch mode and two semi-preparative MIP-SPE columns were individually packed. After investigaing "functional complementation", 2D-MIP-SPE was constructed using ellagic acid MIP and punicalagin MIP-SPE as the first and second dimension, respectively. Then, pomegranate husk extract was fast divided into four fractions individually enriching in ellagic acid, granatin A, punicalagin and ellagic acid glucoside by 2D-MIP-SPE. With the aid of crystallization and RPLC, ellagic acid (13.5 mg) and punicalagin (53.4 mg) were fast obtained in 30 min. Ellagic acid glucoside was purified to the purity near 100% with a recovery of 86.1%. Granatin A (92%) was directly obtained by 2D-MIP-SPE with the recovery of 81.8%. All above indicated that 2D-MIP-SPE was highly efficient in natural product purification. The concept of "functional complementation" was expected to be a useful tool in the construction of 2D-MIP-SPE. (C) 2017 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000403529700003
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152199
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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Sun, Guangying,Liu, Yanfang,Ahat, Hasanjan,et al. "Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1505:35-42.
APA Sun, Guangying.,Liu, Yanfang.,Ahat, Hasanjan.,Shen, Aijin.,Liang, Xinmiao.,...&Aisa, Haji Akber.(2017)."Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract.JOURNAL OF CHROMATOGRAPHY A,1505,35-42.
MLA Sun, Guangying,et al.""Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract".JOURNAL OF CHROMATOGRAPHY A 1505(2017):35-42.
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