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题名: "Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract
作者: Sun, Guangying1, 3;  Liu, Yanfang2;  Ahat, Hasanjan1, 3;  Shen, Aijin2;  Liang, Xinmiao2;  Xue, Xingya2;  Luo, Yuqin1, 3;  Yang, Pan1, 3;  Liu, Zhao-Sheng1, 3;  Aisa, Haji Akber1, 3
关键词: "Two-dimensional" molecularly imprinted solid-phase extraction ;  Functional complementation ;  Crystallization ;  High-performance liquid chromatography ;  Pomegranate husk extract
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2017-07-07
DOI: 10.1016/j.chroma.2017.05.033
卷: 1505, 页:35-42
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, "two dimensional" molecularly imprinted solid-phase extraction (2D-MIP-SPE) of semi preparative grade was constructed to fast purify ellagitannins in pomegranate husk extract with the help of crystallization and reverse-phase liquid chromatgoraphy(RPLC). Ellagic acid and punicalagin imprinted polymers were synthesized in batch mode and two semi-preparative MIP-SPE columns were individually packed. After investigaing "functional complementation", 2D-MIP-SPE was constructed using ellagic acid MIP and punicalagin MIP-SPE as the first and second dimension, respectively. Then, pomegranate husk extract was fast divided into four fractions individually enriching in ellagic acid, granatin A, punicalagin and ellagic acid glucoside by 2D-MIP-SPE. With the aid of crystallization and RPLC, ellagic acid (13.5 mg) and punicalagin (53.4 mg) were fast obtained in 30 min. Ellagic acid glucoside was purified to the purity near 100% with a recovery of 86.1%. Granatin A (92%) was directly obtained by 2D-MIP-SPE with the recovery of 81.8%. All above indicated that 2D-MIP-SPE was highly efficient in natural product purification. The concept of "functional complementation" was expected to be a useful tool in the construction of 2D-MIP-SPE. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: ANTIOXIDANT ACTIVITIES ;  FUNCTIONAL MONOMER ;  WATER SAMPLES ;  ELLAGIC ACID ;  POLYMERS ;  RECOGNITION ;  PRODUCTS ;  NANOPARTICLES ;  TEMPLATES ;  AFFINITY
语种: 英语
WOS记录号: WOS:000403529700003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152199
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Guangying,Liu, Yanfang,Ahat, Hasanjan,et al. "Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1505:35-42.
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