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题名: A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity
作者: Leng, Shuang1, 2;  Qiao, Qinglong1, 3;  Miao, Lu1;  Deng, Wuguo2, 4;  Cui, Jingnan3;  Xu, Zhaochao1
刊名: CHEMICAL COMMUNICATIONS
发表日期: 2017-06-18
DOI: 10.1039/c7cc01483j
卷: 53, 页:6448-6451
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O-6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.
关键词[WOS]: PROTEIN INTERACTIONS ;  SENSOR PROTEINS ;  FLUORESCENCE ;  FLUOROPHORE ;  DYNAMICS ;  CELLS
语种: 英语
WOS记录号: WOS:000403211300015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152216
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Canc Ctr, Inst Canc Stem Cell, Dalian, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
4.Sun Yat Sen Univ, Canc Ctr, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China

Recommended Citation:
Leng, Shuang,Qiao, Qinglong,Miao, Lu,et al. A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity[J]. CHEMICAL COMMUNICATIONS,2017,53:6448-6451.
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