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A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity
Leng, Shuang1,2; Qiao, Qinglong1,3; Miao, Lu1; Deng, Wuguo2,4; Cui, Jingnan3; Xu, Zhaochao1
刊名CHEMICAL COMMUNICATIONS
2017-06-18
DOI10.1039/c7cc01483j
53页:6448-6451
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PROTEIN INTERACTIONS ; SENSOR PROTEINS ; FLUORESCENCE ; FLUOROPHORE ; DYNAMICS ; CELLS
英文摘要A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O-6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.
语种英语
WOS记录号WOS:000403211300015
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152216
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Canc Ctr, Inst Canc Stem Cell, Dalian, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
4.Sun Yat Sen Univ, Canc Ctr, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China
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GB/T 7714
Leng, Shuang,Qiao, Qinglong,Miao, Lu,et al. A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity[J]. CHEMICAL COMMUNICATIONS,2017,53:6448-6451.
APA Leng, Shuang,Qiao, Qinglong,Miao, Lu,Deng, Wuguo,Cui, Jingnan,&Xu, Zhaochao.(2017).A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity.CHEMICAL COMMUNICATIONS,53,6448-6451.
MLA Leng, Shuang,et al."A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity".CHEMICAL COMMUNICATIONS 53(2017):6448-6451.
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