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题名: Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest
作者: Wang, Hongwei1, 2;  Yao, Yating2;  Li, Ya2, 3;  Ma, Shujuan2, 3;  Peng, Xiaojun1;  Ou, Junjie2;  Ye, Mingliang2
关键词: Open tubular column ;  Octaglycidyldimethylsilyl polyhedral oligomeric silsesquioxanes ;  4-aminophenyl disulfide ;  Ring-opening polymerization ;  Capillary liquid chromatography
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2017-08-01
DOI: 10.1016/j.aca.2017.05.004
卷: 979, 页:58-65
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An open tubular (OT) column (25 mu m i.d.) was prepared by in situ ring-opening polymerization of octaglycidyldimethylsilyl polyhedral oligomeric silsesquioxanes (POSS-epoxy) with 4-aminophenyl di-sulfide (APDS) in a binary porogenic system of ethanol/H2O. It was found that porogenic composition played an important role in the formation of OT stationary phases. The ratio of ethanol/H2O at 6/1 (v/v) would lead to the fabrication of hybrid monoliths, while the ratio of ethanol/H2O at 13/1 (v/v) would result in the synthesis of OT phases. In addition, the effects of precursor content and reaction duration on the thickness of OT stationary phases were investigated. Either lower precursor content or shorter reaction duration would produce thinner layer of OT column. The repeatability of OT columns was evaluated through relative standard deviation (RSD%) with benzene as the analyte. The run-to-run, columnto- column and batch-to-batch repeatabilities were 1.7%, 4.8% and 5.6%, respectively, exhibiting satisfactory repeatability of the OT column. Then tryptic digest of mouse liver proteins was used to evaluate the performance of the resulting OT columns (25 mu m i.d. x 2.5 m in length) by cLC-MS/MS analysis, demonstrating their potential in proteome analysis. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  POROUS-LAYER ;  PROTEOMIC ANALYSIS ;  SILICA CAPILLARIES ;  MONOLITHIC COLUMNS ;  KINETIC OPTIMIZATION ;  ORGANIC FRAMEWORKS ;  HIGHLY EFFICIENT ;  LC-MS
语种: 英语
WOS记录号: WOS:000402864100007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152226
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Northwest Univ Xian, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710069, Peoples R China

Recommended Citation:
Wang, Hongwei,Yao, Yating,Li, Ya,et al. Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest[J]. ANALYTICA CHIMICA ACTA,2017,979:58-65.
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