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Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest
Wang, Hongwei1,2; Yao, Yating2; Li, Ya2,3; Ma, Shujuan2,3; Peng, Xiaojun1; Ou, Junjie2; Ye, Mingliang2
KeywordOpen Tubular Column Octaglycidyldimethylsilyl Polyhedral Oligomeric Silsesquioxanes 4-aminophenyl Disulfide Ring-opening Polymerization Capillary Liquid Chromatography
Source PublicationANALYTICA CHIMICA ACTA
2017-08-01
DOI10.1016/j.aca.2017.05.004
Volume979Pages:58-65
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; POROUS-LAYER ; PROTEOMIC ANALYSIS ; SILICA CAPILLARIES ; MONOLITHIC COLUMNS ; KINETIC OPTIMIZATION ; ORGANIC FRAMEWORKS ; HIGHLY EFFICIENT ; LC-MS
AbstractAn open tubular (OT) column (25 mu m i.d.) was prepared by in situ ring-opening polymerization of octaglycidyldimethylsilyl polyhedral oligomeric silsesquioxanes (POSS-epoxy) with 4-aminophenyl di-sulfide (APDS) in a binary porogenic system of ethanol/H2O. It was found that porogenic composition played an important role in the formation of OT stationary phases. The ratio of ethanol/H2O at 6/1 (v/v) would lead to the fabrication of hybrid monoliths, while the ratio of ethanol/H2O at 13/1 (v/v) would result in the synthesis of OT phases. In addition, the effects of precursor content and reaction duration on the thickness of OT stationary phases were investigated. Either lower precursor content or shorter reaction duration would produce thinner layer of OT column. The repeatability of OT columns was evaluated through relative standard deviation (RSD%) with benzene as the analyte. The run-to-run, columnto- column and batch-to-batch repeatabilities were 1.7%, 4.8% and 5.6%, respectively, exhibiting satisfactory repeatability of the OT column. Then tryptic digest of mouse liver proteins was used to evaluate the performance of the resulting OT columns (25 mu m i.d. x 2.5 m in length) by cLC-MS/MS analysis, demonstrating their potential in proteome analysis. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000402864100007
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Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152226
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Northwest Univ Xian, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710069, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hongwei,Yao, Yating,Li, Ya,et al. Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest[J]. ANALYTICA CHIMICA ACTA,2017,979:58-65.
APA Wang, Hongwei.,Yao, Yating.,Li, Ya.,Ma, Shujuan.,Peng, Xiaojun.,...&Ye, Mingliang.(2017).Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest.ANALYTICA CHIMICA ACTA,979,58-65.
MLA Wang, Hongwei,et al."Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest".ANALYTICA CHIMICA ACTA 979(2017):58-65.
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