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题名: A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice
作者: Wang, Li1;  Tao, Tingting1;  Su, Wentao1;  Yu, Hao1;  Yu, Yue1;  Qin, Jianhua1, 2
刊名: LAB ON A CHIP
发表日期: 2017-05-21
DOI: 10.1039/c7lc00134g
卷: 17, 页:1749-1760
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: Diabetic nephropathy is a major chronic renal complication of diabetes mellitus, and is the leading cause of end-stage kidney diseases. Establishing a disease model of diabetic nephropathy in vitro can accelerate the understanding of its mechanisms and pharmaceutical development. We provide the proof-of-principle for using a glomerulus-on-a-chip microdevice that reconstitutes organ-level kidney functions to create a human disease model of early stage diabetic nephropathy on chip. The microfluidic device, which recapitulates the glomerular microenvironment, consists of parallel channels lined by isolated primary glomerular microtissues that experience fluid flow to mimic the glomerular filtration barrier (GFB), including glomerular endothelial cells, 3D basement membrane and podocytes. This device was used to reproduce high glucose-induced critical pathological responses in diabetic nephropathy as observed in humans. The results reveal that hyperglycemia plays a crucial role in the development of increased barrier permeability to albumin and glomerular dysfunction that lead to proteinuria. This organ-on-a-chip microdevice mimics the critical pathological responses of glomerulus that are characteristic of diabetic nephropathy that has not been possible by cell-based and animal models, providing a useful platform for studying the mechanism of diabetic nephropathy and developing an effective therapy in glomerular diseases.
关键词[WOS]: GLUCOSE TRANSPORTERS ;  MICROFLUIDIC DEVICE ;  ENDOTHELIAL-CELLS ;  PODOCYTE ;  ACTIVATION ;  INTERROGATION ;  DYSFUNCTION ;  TECHNOLOGY ;  EXPRESSION ;  CULTURE
语种: 英语
WOS记录号: WOS:000401551300006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152255
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Li,Tao, Tingting,Su, Wentao,et al. A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice[J]. LAB ON A CHIP,2017,17:1749-1760.
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