DICP OpenIR
A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice
Wang, Li1; Tao, Tingting1; Su, Wentao1; Yu, Hao1; Yu, Yue1; Qin, Jianhua1,2
Source PublicationLAB ON A CHIP
2017-05-21
DOI10.1039/c7lc00134g
Volume17Pages:1749-1760
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordGLUCOSE TRANSPORTERS ; MICROFLUIDIC DEVICE ; ENDOTHELIAL-CELLS ; PODOCYTE ; ACTIVATION ; INTERROGATION ; DYSFUNCTION ; TECHNOLOGY ; EXPRESSION ; CULTURE
AbstractDiabetic nephropathy is a major chronic renal complication of diabetes mellitus, and is the leading cause of end-stage kidney diseases. Establishing a disease model of diabetic nephropathy in vitro can accelerate the understanding of its mechanisms and pharmaceutical development. We provide the proof-of-principle for using a glomerulus-on-a-chip microdevice that reconstitutes organ-level kidney functions to create a human disease model of early stage diabetic nephropathy on chip. The microfluidic device, which recapitulates the glomerular microenvironment, consists of parallel channels lined by isolated primary glomerular microtissues that experience fluid flow to mimic the glomerular filtration barrier (GFB), including glomerular endothelial cells, 3D basement membrane and podocytes. This device was used to reproduce high glucose-induced critical pathological responses in diabetic nephropathy as observed in humans. The results reveal that hyperglycemia plays a crucial role in the development of increased barrier permeability to albumin and glomerular dysfunction that lead to proteinuria. This organ-on-a-chip microdevice mimics the critical pathological responses of glomerulus that are characteristic of diabetic nephropathy that has not been possible by cell-based and animal models, providing a useful platform for studying the mechanism of diabetic nephropathy and developing an effective therapy in glomerular diseases.
Language英语
WOS IDWOS:000401551300006
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152255
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Li,Tao, Tingting,Su, Wentao,et al. A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice[J]. LAB ON A CHIP,2017,17:1749-1760.
APA Wang, Li,Tao, Tingting,Su, Wentao,Yu, Hao,Yu, Yue,&Qin, Jianhua.(2017).A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice.LAB ON A CHIP,17,1749-1760.
MLA Wang, Li,et al."A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice".LAB ON A CHIP 17(2017):1749-1760.
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