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A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice
Wang, Li1; Tao, Tingting1; Su, Wentao1; Yu, Hao1; Yu, Yue1; Qin, Jianhua1,2
刊名LAB ON A CHIP
2017-05-21
DOI10.1039/c7lc00134g
17页:1749-1760
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]GLUCOSE TRANSPORTERS ; MICROFLUIDIC DEVICE ; ENDOTHELIAL-CELLS ; PODOCYTE ; ACTIVATION ; INTERROGATION ; DYSFUNCTION ; TECHNOLOGY ; EXPRESSION ; CULTURE
英文摘要Diabetic nephropathy is a major chronic renal complication of diabetes mellitus, and is the leading cause of end-stage kidney diseases. Establishing a disease model of diabetic nephropathy in vitro can accelerate the understanding of its mechanisms and pharmaceutical development. We provide the proof-of-principle for using a glomerulus-on-a-chip microdevice that reconstitutes organ-level kidney functions to create a human disease model of early stage diabetic nephropathy on chip. The microfluidic device, which recapitulates the glomerular microenvironment, consists of parallel channels lined by isolated primary glomerular microtissues that experience fluid flow to mimic the glomerular filtration barrier (GFB), including glomerular endothelial cells, 3D basement membrane and podocytes. This device was used to reproduce high glucose-induced critical pathological responses in diabetic nephropathy as observed in humans. The results reveal that hyperglycemia plays a crucial role in the development of increased barrier permeability to albumin and glomerular dysfunction that lead to proteinuria. This organ-on-a-chip microdevice mimics the critical pathological responses of glomerulus that are characteristic of diabetic nephropathy that has not been possible by cell-based and animal models, providing a useful platform for studying the mechanism of diabetic nephropathy and developing an effective therapy in glomerular diseases.
语种英语
WOS记录号WOS:000401551300006
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152255
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Li,Tao, Tingting,Su, Wentao,et al. A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice[J]. LAB ON A CHIP,2017,17:1749-1760.
APA Wang, Li,Tao, Tingting,Su, Wentao,Yu, Hao,Yu, Yue,&Qin, Jianhua.(2017).A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice.LAB ON A CHIP,17,1749-1760.
MLA Wang, Li,et al."A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice".LAB ON A CHIP 17(2017):1749-1760.
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