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题名: Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis
作者: Liu, Zhongshan1, 2;  Liu, Jing1, 2;  Liu, Zheyi1, 2;  Wang, Hongwei1;  Ou, Junjie1;  Ye, Mingliang1;  Zou, Hanfa1, 2
关键词: Monolithic column ;  Thiol-ene click reaction ;  Proteomics analysis ;  Two-dimensional separation ;  Liquid chromatography
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2017-05-19
DOI: 10.1016/j.chroma.2017.01.029
卷: 1498, 页:29-36
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The vinyl-functionalized hybrid monolithic columns (75 and 150 mu m i.d.) were prepared via solgel chemistry of tetramethoxysilane (TMOS) and vinyltrimethoxysilane (VTMS). The content of accessible vinyl groups was further improved after the monolithic column was post-treated with vinyldimethylethoxysilane (VDMES). The surface properties of monolithic columns were tailored via thiol-ene click reaction by using 1-octadecanethiol, sodium 3-mercapto-l-propanesulfonate and 2,2'-(ethylenedioxy)diethanethiol/vinylphosphonic acid, respectively. The preparing octadecylfunctionalized monolithic columns were adopted for proteomics analysis in cLC-MS/MS. A 37-cm-long x 75-mu m-i.d. monolithic column could identify 3918 unique peptides and 1067 unique proteins in the tryptic digest of proteins from HeLa cells. When a 90-cm-long x 75-mu m-i.d. monolithic column was used, the numbers of unique peptides and proteins were increased by 82% and 32%, respectively. Furthermore, strong cation exchange (SCX) monolithic columns (4 cm in length x 150 mu m i.d.) were also prepared and coupled with the 37-cm-long x 75-mu m-i.d. octadecyl-functionalized monolithic column for two-dimensional" SCX-RPLC-MS/MS analysis, which could identify 17114 unique peptides and 3211 unique proteins. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  CATION-EXCHANGE MONOLITH ;  TANDEM MASS-SPECTROMETRY ;  ONE-POT PROCESS ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  NANOELECTROSPRAY IONIZATION ;  FACILE PREPARATION ;  GRADIENT ELUTION ;  SILICA MONOLITHS ;  SEPARATION
语种: 英语
WOS记录号: WOS:000401043000005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152264
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Zhongshan,Liu, Jing,Liu, Zheyi,et al. Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:29-36.
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