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Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis
Liu, Zhongshan1,2; Liu, Jing1,2; Liu, Zheyi1,2; Wang, Hongwei1; Ou, Junjie1; Ye, Mingliang1; Zou, Hanfa1,2
KeywordMonolithic Column Thiol-ene Click Reaction Proteomics Analysis Two-dimensional Separation Liquid Chromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2017-05-19
DOI10.1016/j.chroma.2017.01.029
Volume1498Pages:29-36
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; CATION-EXCHANGE MONOLITH ; TANDEM MASS-SPECTROMETRY ; ONE-POT PROCESS ; CAPILLARY ELECTROCHROMATOGRAPHY ; NANOELECTROSPRAY IONIZATION ; FACILE PREPARATION ; GRADIENT ELUTION ; SILICA MONOLITHS ; SEPARATION
AbstractThe vinyl-functionalized hybrid monolithic columns (75 and 150 mu m i.d.) were prepared via solgel chemistry of tetramethoxysilane (TMOS) and vinyltrimethoxysilane (VTMS). The content of accessible vinyl groups was further improved after the monolithic column was post-treated with vinyldimethylethoxysilane (VDMES). The surface properties of monolithic columns were tailored via thiol-ene click reaction by using 1-octadecanethiol, sodium 3-mercapto-l-propanesulfonate and 2,2'-(ethylenedioxy)diethanethiol/vinylphosphonic acid, respectively. The preparing octadecylfunctionalized monolithic columns were adopted for proteomics analysis in cLC-MS/MS. A 37-cm-long x 75-mu m-i.d. monolithic column could identify 3918 unique peptides and 1067 unique proteins in the tryptic digest of proteins from HeLa cells. When a 90-cm-long x 75-mu m-i.d. monolithic column was used, the numbers of unique peptides and proteins were increased by 82% and 32%, respectively. Furthermore, strong cation exchange (SCX) monolithic columns (4 cm in length x 150 mu m i.d.) were also prepared and coupled with the 37-cm-long x 75-mu m-i.d. octadecyl-functionalized monolithic column for two-dimensional" SCX-RPLC-MS/MS analysis, which could identify 17114 unique peptides and 3211 unique proteins. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000401043000005
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152264
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zhongshan,Liu, Jing,Liu, Zheyi,et al. Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:29-36.
APA Liu, Zhongshan.,Liu, Jing.,Liu, Zheyi.,Wang, Hongwei.,Ou, Junjie.,...&Zou, Hanfa.(2017).Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis.JOURNAL OF CHROMATOGRAPHY A,1498,29-36.
MLA Liu, Zhongshan,et al."Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis".JOURNAL OF CHROMATOGRAPHY A 1498(2017):29-36.
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