DICP OpenIR
In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis
Zhao, Qun1; Fang, Fei1,2; Shan, Yichu1; Sui, Zhigang1; Zhao, Baofeng1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
Source PublicationANALYTICAL CHEMISTRY
2017-05-16
DOI10.1021/acs.analchem.6b04232
Volume89Pages:5179-5185
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSAMPLE PREPARATION METHOD ; TANDEM MASS-SPECTROMETRY ; INTEGRATED APPROACH ; HIGH-PH ; QUANTIFICATION ; PROTEINS ; FRACTIONATION ; IDENTIFICATION ; PRETREATMENT ; COMBINATION
AbstractAlthough great achievement has been made in the mapping of human proteome, the efficiency of sample preparation still needs to be improved, especially for membrane proteins. Herein, we presented a novel method to deepen proteome coverage by the sequential extraction of proteins using urea and 1-dodecy1-3- methylimidazolium chloride (C12Im-Cl). With such a strategy, the commonly lost hydrophobic proteins by 8 M urea extraction could be further recovered by C12Im-Cl, as well as the suppression effect of high abundance soluble proteins could be decreased. Followed by the in situ sample preparation and separation with different stationary phases, more than 9810 gene products could be identified, covering 8 orders of magnitude in abundance, which was, to the best of our knowledge, the largest data set of HeLa cell proteome. Compared with previous work, not only the number of proteins identified was obviously increased, but also the analysis time was shortened to a few days. Therefore, we expect that such a strategy has great potential applications to achieve unprecedented coverage for proteome analysis.
Language英语
WOS IDWOS:000401674800003
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152272
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Qun,Fang, Fei,Shan, Yichu,et al. In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2017,89:5179-5185.
APA Zhao, Qun.,Fang, Fei.,Shan, Yichu.,Sui, Zhigang.,Zhao, Baofeng.,...&Zhang, Yukui.(2017).In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis.ANALYTICAL CHEMISTRY,89,5179-5185.
MLA Zhao, Qun,et al."In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis".ANALYTICAL CHEMISTRY 89(2017):5179-5185.
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