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题名: In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis
作者: Zhao, Qun1;  Fang, Fei1, 2;  Shan, Yichu1;  Sui, Zhigang1;  Zhao, Baofeng1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-05-16
DOI: 10.1021/acs.analchem.6b04232
卷: 89, 页:5179-5185
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Although great achievement has been made in the mapping of human proteome, the efficiency of sample preparation still needs to be improved, especially for membrane proteins. Herein, we presented a novel method to deepen proteome coverage by the sequential extraction of proteins using urea and 1-dodecy1-3- methylimidazolium chloride (C12Im-Cl). With such a strategy, the commonly lost hydrophobic proteins by 8 M urea extraction could be further recovered by C12Im-Cl, as well as the suppression effect of high abundance soluble proteins could be decreased. Followed by the in situ sample preparation and separation with different stationary phases, more than 9810 gene products could be identified, covering 8 orders of magnitude in abundance, which was, to the best of our knowledge, the largest data set of HeLa cell proteome. Compared with previous work, not only the number of proteins identified was obviously increased, but also the analysis time was shortened to a few days. Therefore, we expect that such a strategy has great potential applications to achieve unprecedented coverage for proteome analysis.
关键词[WOS]: SAMPLE PREPARATION METHOD ;  TANDEM MASS-SPECTROMETRY ;  INTEGRATED APPROACH ;  HIGH-PH ;  QUANTIFICATION ;  PROTEINS ;  FRACTIONATION ;  IDENTIFICATION ;  PRETREATMENT ;  COMBINATION
语种: 英语
WOS记录号: WOS:000401674800003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152272
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Zhao, Qun,Fang, Fei,Shan, Yichu,et al. In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2017,89:5179-5185.
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