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题名: Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides
作者: Sun, Xudong1;  Dong, Jing2;  Li, Jinan2;  Ye, Mingliang2;  Zhang, Weibing1;  Ou, Junjie2
关键词: Glycopeptides ;  Macroporous adsorption resin ;  Layer-by-layer ;  Hydrophilic interaction liquid ;  chromatography ;  Solid-phase extraction
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2017-05-19
DOI: 10.1016/j.chroma.2016.12.045
卷: 1498, 页:72-79
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A facile approach for preparation of hydrophilic polysaccharide-functionalized macroporous adsorption resin (MAR) was presented. Polydisperse MAR with approximately 250 mu m in diameter was synthesized via suspension polymerization and then modified with polysaccharides, sodium hyaluronate (HA) and chitosan (CS), through layer-by-layer (LbL) self-assembly pro cess. The preparation method was facile, low cost and easy to expand production from tens of grams to one kilogram. The resulting MAR@(HA/CS)(20) possesses highly hydrophilic property and rapid adsorption behavior. The specificity and efficiency of MAR@(HA/CS)(20) to glycopeptide was demonstrated by trapping N-linked glycopeptide from tryptic digests of human immunoglobulin G (IgG) and horseradish peroxidase (HRP) with a homemade solid-phase extraction (SPE) column. The detection sensitivity for glycopeptide determined by MALDI-TOF MS was as low as 5 fmol for standard tryptic digest of human IgG. The enrichment recoveries were higher than 73%. Furthermore, in the analysis of real biological sample, 745 unique N-glycosylation sites in 1353 unique glycopeptides mapped to 379 N-glycosylated proteins were identified in three replicate analyses of protein sample extracted from mouse liver, showing the great potential of MAR@(HA/CS)(20) in the enrichment and identification of low-abundance N-linked glycopeptides in complicated biological samples. (C) 2016 Elsevier B.V. All rights reserved.
关键词[WOS]: N-LINKED GLYCOPROTEINS ;  MAGNETIC NANOPARTICLES ;  MASS-SPECTROMETRY ;  PROTEIN GLYCOSYLATION ;  EFFICIENT ENRICHMENT ;  SEPARATION ;  CHROMATOGRAPHY ;  CAPTURE ;  PURIFICATION ;  AMINO
语种: 英语
WOS记录号: WOS:000401043000010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152275
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Sun, Xudong,Dong, Jing,Li, Jinan,et al. Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:72-79.
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