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Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides
Sun, Xudong1; Dong, Jing2; Li, Jinan2; Ye, Mingliang2; Zhang, Weibing1; Ou, Junjie2
关键词Glycopeptides Macroporous Adsorption Resin Layer-by-layer Hydrophilic Interaction Liquid Chromatography Solid-phase Extraction
刊名JOURNAL OF CHROMATOGRAPHY A
2017-05-19
DOI10.1016/j.chroma.2016.12.045
1498页:72-79
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]N-LINKED GLYCOPROTEINS ; MAGNETIC NANOPARTICLES ; MASS-SPECTROMETRY ; PROTEIN GLYCOSYLATION ; EFFICIENT ENRICHMENT ; SEPARATION ; CHROMATOGRAPHY ; CAPTURE ; PURIFICATION ; AMINO
英文摘要A facile approach for preparation of hydrophilic polysaccharide-functionalized macroporous adsorption resin (MAR) was presented. Polydisperse MAR with approximately 250 mu m in diameter was synthesized via suspension polymerization and then modified with polysaccharides, sodium hyaluronate (HA) and chitosan (CS), through layer-by-layer (LbL) self-assembly pro cess. The preparation method was facile, low cost and easy to expand production from tens of grams to one kilogram. The resulting MAR@(HA/CS)(20) possesses highly hydrophilic property and rapid adsorption behavior. The specificity and efficiency of MAR@(HA/CS)(20) to glycopeptide was demonstrated by trapping N-linked glycopeptide from tryptic digests of human immunoglobulin G (IgG) and horseradish peroxidase (HRP) with a homemade solid-phase extraction (SPE) column. The detection sensitivity for glycopeptide determined by MALDI-TOF MS was as low as 5 fmol for standard tryptic digest of human IgG. The enrichment recoveries were higher than 73%. Furthermore, in the analysis of real biological sample, 745 unique N-glycosylation sites in 1353 unique glycopeptides mapped to 379 N-glycosylated proteins were identified in three replicate analyses of protein sample extracted from mouse liver, showing the great potential of MAR@(HA/CS)(20) in the enrichment and identification of low-abundance N-linked glycopeptides in complicated biological samples. (C) 2016 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000401043000010
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152275
专题中国科学院大连化学物理研究所
作者单位1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Sun, Xudong,Dong, Jing,Li, Jinan,et al. Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:72-79.
APA Sun, Xudong,Dong, Jing,Li, Jinan,Ye, Mingliang,Zhang, Weibing,&Ou, Junjie.(2017).Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1498,72-79.
MLA Sun, Xudong,et al."Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1498(2017):72-79.
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