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题名: Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
作者: Yu, Tao1, 2;  Zhou, Yongjin J.1, 2, 6;  Wenning, Leonie1, 2;  Liu, Quanli1, 2;  Krivoruchko, Anastasia1, 2, 3;  Siewers, Verena1, 2;  Nielsen, Jens1, 2, 4, 5;  David, Florian1, 2, 3
刊名: NATURE COMMUNICATIONS
发表日期: 2017-05-26
DOI: 10.1038/ncomms15587
卷: 8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l(-1) in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.
关键词[WOS]: GENETIC MODIFICATIONS ;  ACETYL-COENZYME ;  JOJOBA OIL ;  YEAST ;  PATHWAY ;  ELONGATION ;  BIOSYNTHESIS ;  SYNTHASE ;  ARABIDOPSIS ;  REDUCTASES
语种: 英语
WOS记录号: WOS:000402047300001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152292
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chalmers, Dept Biol & Biol Engn, Kemivagen 10, SE-41296 Gothenburg, Sweden
2.Chalmers, Novo Nordisk Fdn, Ctr Biosustainabil, SE-41296 Gothenburg, Sweden
3.Chalmers, Biopetrolia AB, Syst & Synthet Biol Grp, Kemivagen 10, SE-41296 Gothenburg, Sweden
4.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark
5.Royal Inst Technol, Sci Life Lab, SE-17121 Stockholm, Sweden
6.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Yu, Tao,Zhou, Yongjin J.,Wenning, Leonie,et al. Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals[J]. NATURE COMMUNICATIONS,2017,8.
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