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Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
Yu, Tao1,2; Zhou, Yongjin J.1,2,6; Wenning, Leonie1,2; Liu, Quanli1,2; Krivoruchko, Anastasia1,2,3; Siewers, Verena1,2; Nielsen, Jens1,2,4,5; David, Florian1,2,3
Source PublicationNATURE COMMUNICATIONS
2017-05-26
DOI10.1038/ncomms15587
Volume8
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordGENETIC MODIFICATIONS ; ACETYL-COENZYME ; JOJOBA OIL ; YEAST ; PATHWAY ; ELONGATION ; BIOSYNTHESIS ; SYNTHASE ; ARABIDOPSIS ; REDUCTASES
AbstractProduction of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l(-1) in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.
Language英语
WOS IDWOS:000402047300001
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152292
Collection中国科学院大连化学物理研究所
Affiliation1.Chalmers, Dept Biol & Biol Engn, Kemivagen 10, SE-41296 Gothenburg, Sweden
2.Chalmers, Novo Nordisk Fdn, Ctr Biosustainabil, SE-41296 Gothenburg, Sweden
3.Chalmers, Biopetrolia AB, Syst & Synthet Biol Grp, Kemivagen 10, SE-41296 Gothenburg, Sweden
4.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark
5.Royal Inst Technol, Sci Life Lab, SE-17121 Stockholm, Sweden
6.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yu, Tao,Zhou, Yongjin J.,Wenning, Leonie,et al. Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals[J]. NATURE COMMUNICATIONS,2017,8.
APA Yu, Tao.,Zhou, Yongjin J..,Wenning, Leonie.,Liu, Quanli.,Krivoruchko, Anastasia.,...&David, Florian.(2017).Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals.NATURE COMMUNICATIONS,8.
MLA Yu, Tao,et al."Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals".NATURE COMMUNICATIONS 8(2017).
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