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题名: Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography
作者: Liu, Shengju1, 3;  Peng, Jiaxi1, 3;  Zhang, Hongyan1, 3;  Li, Xin1, 3;  Liu, Zheyi1, 3;  Kang, Xiaohui1;  Wu, Minghuo2;  Wu, Ren'an1
关键词: Mesoporous carbon nanoparticles ;  Organic-silica hybrid monolithic column ;  Capillary liquid chromatography (cLC) ;  Small molecule ;  Protein ;  Peptide
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2017-05-19
DOI: 10.1016/j.chroma.2017.03.067
卷: 1498, 页:64-71
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An organic-silica hybrid monolithic capillary column was fabricated by crosslinking (3-aminopropyl)trimethoxysilane (APTMS) modified mesoporous carbon nanoparticles (AP-MCNs) with tetramethoxysilane (TMOS) and n-butyltrimethoxysilane (C4-TriMOS). Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, mercury intrusion porosimetry and inverse size-exclusion chromatography characterization proved the successful immobilization of mesoporous carbon nanoparticles (MCNs). The crosslinking of AP-MCNs into the hybrid monolithic matrix has significantly increased the reversed-phase retention of alkylbenzenes and chromatographic performance for small molecules separations in comparison with the neat one without MCNs. The resulting column efficiency of the mesoporous carbon nanoparticle-based butyl-silica hybrid monolithic column (MCN-C4-monolith) was up to ca. 116,600 N/m for the capillary liquid chromatography (cLC) separation of butylbenzene. Enhanced performance of proteins separation was achieved on the MCN-C4-monolith in comparison with the butyl-silica hybrid monolithic column without MCN (C4-monolith). The separation of peptides from bovine serum albumin (BSA) digest was carried out on the MCN-C4-monolith by capillary liquid chromatography-tandem mass spectrometry (cLC-MS/MS) with protein sequence coverage of 81.9%, suggesting its potential application in proteomics. (C) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: ONE-POT PROCESS ;  REVERSED-PHASE CHROMATOGRAPHY ;  SIZE-EXCLUSION CHROMATOGRAPHY ;  SMALL MOLECULES ;  HIGH-EFFICIENCY ;  STATIONARY PHASES ;  PROTEOME ANALYSIS ;  FACILE SYNTHESIS ;  SEPARATION ;  PROTEINS
语种: 英语
WOS记录号: WOS:000401043000009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152294
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Food & Environm, Panjin 124221, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Shengju,Peng, Jiaxi,Zhang, Hongyan,et al. Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:64-71.
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