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Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography
Liu, Shengju1,3; Peng, Jiaxi1,3; Zhang, Hongyan1,3; Li, Xin1,3; Liu, Zheyi1,3; Kang, Xiaohui1; Wu, Minghuo2; Wu, Ren'an1
KeywordMesoporous Carbon Nanoparticles Organic-silica Hybrid Monolithic Column Capillary Liquid Chromatography (Clc) Small Molecule Protein Peptide
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2017-05-19
DOI10.1016/j.chroma.2017.03.067
Volume1498Pages:64-71
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordONE-POT PROCESS ; REVERSED-PHASE CHROMATOGRAPHY ; SIZE-EXCLUSION CHROMATOGRAPHY ; SMALL MOLECULES ; HIGH-EFFICIENCY ; STATIONARY PHASES ; PROTEOME ANALYSIS ; FACILE SYNTHESIS ; SEPARATION ; PROTEINS
AbstractAn organic-silica hybrid monolithic capillary column was fabricated by crosslinking (3-aminopropyl)trimethoxysilane (APTMS) modified mesoporous carbon nanoparticles (AP-MCNs) with tetramethoxysilane (TMOS) and n-butyltrimethoxysilane (C4-TriMOS). Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, mercury intrusion porosimetry and inverse size-exclusion chromatography characterization proved the successful immobilization of mesoporous carbon nanoparticles (MCNs). The crosslinking of AP-MCNs into the hybrid monolithic matrix has significantly increased the reversed-phase retention of alkylbenzenes and chromatographic performance for small molecules separations in comparison with the neat one without MCNs. The resulting column efficiency of the mesoporous carbon nanoparticle-based butyl-silica hybrid monolithic column (MCN-C4-monolith) was up to ca. 116,600 N/m for the capillary liquid chromatography (cLC) separation of butylbenzene. Enhanced performance of proteins separation was achieved on the MCN-C4-monolith in comparison with the butyl-silica hybrid monolithic column without MCN (C4-monolith). The separation of peptides from bovine serum albumin (BSA) digest was carried out on the MCN-C4-monolith by capillary liquid chromatography-tandem mass spectrometry (cLC-MS/MS) with protein sequence coverage of 81.9%, suggesting its potential application in proteomics. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000401043000009
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152294
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Sch Food & Environm, Panjin 124221, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Shengju,Peng, Jiaxi,Zhang, Hongyan,et al. Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:64-71.
APA Liu, Shengju.,Peng, Jiaxi.,Zhang, Hongyan.,Li, Xin.,Liu, Zheyi.,...&Wu, Ren'an.(2017).Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1498,64-71.
MLA Liu, Shengju,et al."Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1498(2017):64-71.
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