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Epitope molecularly imprinted polymer coated quartz crystal microbalance sensor for the determination of human serum albumin
Ma, Xiao-Tong1; He, Xi-Wen1; Li, Wen-You1,2; Zhang, Yu-Kui1,3
关键词Epitope Imprinting Quartz Crystal Microbalance Protein Detection Human Serum Albumin
刊名SENSORS AND ACTUATORS B-CHEMICAL
2017-07-01
DOI10.1016/j.snb.2017.02.137
246页:879-886
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]SELECTIVE DETERMINATION ; PROTEIN RECOGNITION ; IMMUNOGLOBULIN-G ; QCM SENSOR ; SURFACE ; FILMS ; NANOPARTICLES ; ACID ; MACROMOLECULES ; DISCRIMINATION
英文摘要Quantitative determination of biomacromolecules has great significance. Quartz crystal microbalance sensors are favored by their sensibility while their selectivity was limited. Molecularly imprinted polymer has great selectivity performance. By combining the advantages of quartz crystal microbalance and molecularly imprinted polymer, this work established an epitope molecularly imprinted polymer based quartz crystal microbalance sensor. Using the epitope of human serum albumin as the template, the epitope molecularly imprinted polymer (EMIP) was successfully synthesized. Then a simple coating method was applied to fabricate the epitope molecularly imprinted polymer based quartz crystal microbalance sensor (EMIP-QCM). The EMIP-QCM sensor had good sensibility and selectivity to the target human serum albumin. The linear range was from 0.050u.,mu gmL(-1) to 0.500 mu gmL(-1) and the detection limit was calculated to be 0.026 mu g mL(-1). In addition, it could be used in real sample analysis and had good accuracy and reproducibility. This method proposed a general and simple way of establishing quartz crystal microbalance sensors for sensitive determination of biomacromolecules. (C) 2017 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000400803700107
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152296
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,et al. Epitope molecularly imprinted polymer coated quartz crystal microbalance sensor for the determination of human serum albumin[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,246:879-886.
APA Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2017).Epitope molecularly imprinted polymer coated quartz crystal microbalance sensor for the determination of human serum albumin.SENSORS AND ACTUATORS B-CHEMICAL,246,879-886.
MLA Ma, Xiao-Tong,et al."Epitope molecularly imprinted polymer coated quartz crystal microbalance sensor for the determination of human serum albumin".SENSORS AND ACTUATORS B-CHEMICAL 246(2017):879-886.
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