DICP OpenIR
Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device
Li, Zhongyu1,2; Jiang, Lei1; Tao, Tingting1; Su, Wentao1; Guo, Yaqiong1,2; Yu, Hao1; Qin, Jianhua1,2
刊名TOXICOLOGY RESEARCH
2017-05-01
DOI10.1039/c6tx00417b
6页:372-380
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]DRUG-INDUCED NEPHROTOXICITY ; OXIDATIVE STRESS ; CELL-CULTURE ; IN-VITRO ; TOXICITY ; APOPTOSIS ; EXPOSURE ; PATHWAY ; LIVER ; ACTIVATION
英文摘要Cadmium (Cd) is a common environmental pollutant. Its effects on human health have attracted great attention. The kidney is the organ that is the most affected by Cd exposure. Thus, it is highly desirable to develop a reliable model to evaluate Cd-induced nephrotoxicity in vitro. We present a kidney-on-a-chip with three compartmentalized culture chambers to examine Cd-induced nephrotoxicity. The culture and collection channels represent the capillary and the glomerular capsule sides of the glomerular filtration barrier, respectively. Isolated primary rat glomerular endothelial cells (GECs) were cultured on the side surface of the middle gel channel. The integrated GEC layer demonstrated the selective permeability of the renal barrier. Therefore, it was further utilized to study the nephrotoxicity induced by Cd exposure at different concentrations. Cd induced significant cytotoxicity and disrupted the expression of tight junction protein ZO-1 in a dose-dependent manner. Moreover, Cd exposure increased the permeability of the endothelial layer to large molecules, immunoglobulin G and albumin. These results facilitate the understanding of the underlying mechanism of kidney dysfunction and glomerular disease. This is the first study on Cd-induced nephrotoxicity using primary GECs in a microfluidic device. The kidney-on-a-chip device enables direct visualization and quantitative analysis of GEC responses to Cd in real time. It may provide a micro-scale platform based on the human system for nephrotoxicity testing under varying environmental exposure.
语种英语
WOS记录号WOS:000401000400012
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152300
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li, Zhongyu,Jiang, Lei,Tao, Tingting,et al. Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device[J]. TOXICOLOGY RESEARCH,2017,6:372-380.
APA Li, Zhongyu.,Jiang, Lei.,Tao, Tingting.,Su, Wentao.,Guo, Yaqiong.,...&Qin, Jianhua.(2017).Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device.TOXICOLOGY RESEARCH,6,372-380.
MLA Li, Zhongyu,et al."Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device".TOXICOLOGY RESEARCH 6(2017):372-380.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Zhongyu]的文章
[Jiang, Lei]的文章
[Tao, Tingting]的文章
百度学术
百度学术中相似的文章
[Li, Zhongyu]的文章
[Jiang, Lei]的文章
[Tao, Tingting]的文章
必应学术
必应学术中相似的文章
[Li, Zhongyu]的文章
[Jiang, Lei]的文章
[Tao, Tingting]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。