DICP OpenIR
Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device
Li, Zhongyu1,2; Jiang, Lei1; Tao, Tingting1; Su, Wentao1; Guo, Yaqiong1,2; Yu, Hao1; Qin, Jianhua1,2
Source PublicationTOXICOLOGY RESEARCH
2017-05-01
DOI10.1039/c6tx00417b
Volume6Pages:372-380
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectToxicology
WOS Research AreaToxicology
WOS KeywordDRUG-INDUCED NEPHROTOXICITY ; OXIDATIVE STRESS ; CELL-CULTURE ; IN-VITRO ; TOXICITY ; APOPTOSIS ; EXPOSURE ; PATHWAY ; LIVER ; ACTIVATION
AbstractCadmium (Cd) is a common environmental pollutant. Its effects on human health have attracted great attention. The kidney is the organ that is the most affected by Cd exposure. Thus, it is highly desirable to develop a reliable model to evaluate Cd-induced nephrotoxicity in vitro. We present a kidney-on-a-chip with three compartmentalized culture chambers to examine Cd-induced nephrotoxicity. The culture and collection channels represent the capillary and the glomerular capsule sides of the glomerular filtration barrier, respectively. Isolated primary rat glomerular endothelial cells (GECs) were cultured on the side surface of the middle gel channel. The integrated GEC layer demonstrated the selective permeability of the renal barrier. Therefore, it was further utilized to study the nephrotoxicity induced by Cd exposure at different concentrations. Cd induced significant cytotoxicity and disrupted the expression of tight junction protein ZO-1 in a dose-dependent manner. Moreover, Cd exposure increased the permeability of the endothelial layer to large molecules, immunoglobulin G and albumin. These results facilitate the understanding of the underlying mechanism of kidney dysfunction and glomerular disease. This is the first study on Cd-induced nephrotoxicity using primary GECs in a microfluidic device. The kidney-on-a-chip device enables direct visualization and quantitative analysis of GEC responses to Cd in real time. It may provide a micro-scale platform based on the human system for nephrotoxicity testing under varying environmental exposure.
Language英语
WOS IDWOS:000401000400012
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152300
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Zhongyu,Jiang, Lei,Tao, Tingting,et al. Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device[J]. TOXICOLOGY RESEARCH,2017,6:372-380.
APA Li, Zhongyu.,Jiang, Lei.,Tao, Tingting.,Su, Wentao.,Guo, Yaqiong.,...&Qin, Jianhua.(2017).Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device.TOXICOLOGY RESEARCH,6,372-380.
MLA Li, Zhongyu,et al."Assessment of cadmium-induced nephrotoxicity using a kidney-on-a-chip device".TOXICOLOGY RESEARCH 6(2017):372-380.
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