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An immobilized titanium (IV) ion affinity chromatography adsorbent for solid phase extraction of phosphopeptides for phosphoproteome analysis
Yao, Yating1,2; Dong, Jing1; Dong, Mingming1,2; Liu, Fangjie1,2; Wang, Yan1,2; Mao, Jiawei1,2; Ye, Mingliang1; Zou, Hanfa1
KeywordProtein Phosphorylation Immobilized Titanium (Iv) Ion Affinity Chromatography Solid Phase Extraction Phosphopeptide Enrichment Liquid Chromatography-tandem Mass Spectrometry
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2017-05-19
DOI10.1016/j.chroma.2017.03.026
Volume1498Pages:22-28
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMASS-SPECTROMETRY ; PHOSPHORYLATED PEPTIDES ; ENRICHMENT ; CANCER ; IMAC ; RESOLUTION ; DYNAMICS ; ROBUST ; CELLS
AbstractIn this study, we developed a centrifugation assisted solid phase extraction (SPE) method for the selective enrichment of phosphopeptides using a new Ti4+-IMAC material synthesized in-house. This new material has the feature of big size and large specific surface area which makes it more suitable to enrich phosphopeptides in a SPE way. The spin tips loaded with the Ti4+-IMAC material were applied to enrich phosphopeptides from the complex protein digests. It was found that phosphopeptides can be specifically enriched from tryptic digest of bovine serum albumin and 3-casein at a molar ratio up to 1000:1. And about 4700 unique phosphorylated peptides can be identified with the specificity as high as 99% from the tryptic digest of HeLa cell proteins. This tip was demonstrated to have good column-to-column reproducibility. Furthermore, it is fitted to analyze minute amount of sample. Compared with the conventional solution method, the SPE method facilitated the rapid and complete separation of the material with solution, which making it a time-saving and convenient method for phosphopeptide enrichment. Compared with the commercial TiO2 material, this new materials yielded much more phosphopeptide identifications and much higher enrichment specificity. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000401043000004
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152310
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yao, Yating,Dong, Jing,Dong, Mingming,et al. An immobilized titanium (IV) ion affinity chromatography adsorbent for solid phase extraction of phosphopeptides for phosphoproteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:22-28.
APA Yao, Yating.,Dong, Jing.,Dong, Mingming.,Liu, Fangjie.,Wang, Yan.,...&Zou, Hanfa.(2017).An immobilized titanium (IV) ion affinity chromatography adsorbent for solid phase extraction of phosphopeptides for phosphoproteome analysis.JOURNAL OF CHROMATOGRAPHY A,1498,22-28.
MLA Yao, Yating,et al."An immobilized titanium (IV) ion affinity chromatography adsorbent for solid phase extraction of phosphopeptides for phosphoproteome analysis".JOURNAL OF CHROMATOGRAPHY A 1498(2017):22-28.
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