DICP OpenIR
Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine
Wan, Lingzhong1; Zhu, Haijing1; Guan, Yafeng2; Huang, Guangming1
KeywordNanocoating Cellulose Paper Based Microextraction Zirconia Nanospray Mass Spectrometry Ribonucleosides
Source PublicationTALANTA
2017-07-01
DOI10.1016/j.talanta.2017.03.085
Volume169Pages:209-215
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordLIQUID-CHROMATOGRAPHY ; BREAST-CANCER ; RIBOSYLATED METABOLITES ; SELECTIVE ENRICHMENT ; MODIFIED NUCLEOSIDES ; STATIONARY PHASES ; ZIRCONIA ; TITANIA ; IDENTIFICATION ; NANOPARTICLES
AbstractA rapid and facile analytical method for quantification of ribonucleosides in human urine was developed by the combination of nanocoating cellulose paper based microextraction and nanoelectrospray ionization-tandem mass spectrometry (nESI-MS/MS). Cellulose paper used for microextraction was modified by nano-precision deposition of uniform ultrathin zirconia gel film using a sol-gel process. Due to the large surface area of the cellulose paper and the strong affinity between zirconia and the cis-diol compounds, the target analytes were selectively extracted from the complex matrix. Thus, the detection sensitivity was greatly improved. Typically, the nanocoating cellulose paper was immersed into the diluted urine for selective extraction of target analytes, then the extracted analytes were subjected to nESI-MS/MS detection. The whole analytical procedure could be completed within 10 min. The method was evaluated by the determination of ribonucleosides (adenosine, cytidine, uridine, guanosine) in urine sample. The signal intensities of the ribonuclesides extracted by the nanocoating cellulose paper were greatly enhanced by 136-459-folds compared with the one of the unmodified cellulose paper based microextraction. The limits of detection (LODs) and the limits of quantification (LOQs) of the four ribonucleosides were in the range of 0.0136-1.258 mu g L-1 and 0.0454-4.194 mu g L-1, respectively. The recoveries of the target nucleosides from spiked human urine were in the range of 75.64-103.49% with the relative standard deviations (RSDs) less than 9.36%. The results demonstrate the potential of the proposed method for rapid and facile determination of endogenous ribonucleosides in urine sample.
Language英语
WOS IDWOS:000401206700030
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152314
Collection中国科学院大连化学物理研究所
Affiliation1.Univ Sci & Technol China, Dept Chem, Sch Chem & Mat Sci, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wan, Lingzhong,Zhu, Haijing,Guan, Yafeng,et al. Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine[J]. TALANTA,2017,169:209-215.
APA Wan, Lingzhong,Zhu, Haijing,Guan, Yafeng,&Huang, Guangming.(2017).Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine.TALANTA,169,209-215.
MLA Wan, Lingzhong,et al."Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine".TALANTA 169(2017):209-215.
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