中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine
作者: Wan, Lingzhong1;  Zhu, Haijing1;  Guan, Yafeng2;  Huang, Guangming1
关键词: Nanocoating cellulose paper based microextraction ;  Zirconia ;  Nanospray mass spectrometry ;  Ribonucleosides
刊名: TALANTA
发表日期: 2017-07-01
DOI: 10.1016/j.talanta.2017.03.085
卷: 169, 页:209-215
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A rapid and facile analytical method for quantification of ribonucleosides in human urine was developed by the combination of nanocoating cellulose paper based microextraction and nanoelectrospray ionization-tandem mass spectrometry (nESI-MS/MS). Cellulose paper used for microextraction was modified by nano-precision deposition of uniform ultrathin zirconia gel film using a sol-gel process. Due to the large surface area of the cellulose paper and the strong affinity between zirconia and the cis-diol compounds, the target analytes were selectively extracted from the complex matrix. Thus, the detection sensitivity was greatly improved. Typically, the nanocoating cellulose paper was immersed into the diluted urine for selective extraction of target analytes, then the extracted analytes were subjected to nESI-MS/MS detection. The whole analytical procedure could be completed within 10 min. The method was evaluated by the determination of ribonucleosides (adenosine, cytidine, uridine, guanosine) in urine sample. The signal intensities of the ribonuclesides extracted by the nanocoating cellulose paper were greatly enhanced by 136-459-folds compared with the one of the unmodified cellulose paper based microextraction. The limits of detection (LODs) and the limits of quantification (LOQs) of the four ribonucleosides were in the range of 0.0136-1.258 mu g L-1 and 0.0454-4.194 mu g L-1, respectively. The recoveries of the target nucleosides from spiked human urine were in the range of 75.64-103.49% with the relative standard deviations (RSDs) less than 9.36%. The results demonstrate the potential of the proposed method for rapid and facile determination of endogenous ribonucleosides in urine sample.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  BREAST-CANCER ;  RIBOSYLATED METABOLITES ;  SELECTIVE ENRICHMENT ;  MODIFIED NUCLEOSIDES ;  STATIONARY PHASES ;  ZIRCONIA ;  TITANIA ;  IDENTIFICATION ;  NANOPARTICLES
语种: 英语
WOS记录号: WOS:000401206700030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152314
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Sci & Technol China, Dept Chem, Sch Chem & Mat Sci, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China

Recommended Citation:
Wan, Lingzhong,Zhu, Haijing,Guan, Yafeng,et al. Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine[J]. TALANTA,2017,169:209-215.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wan, Lingzhong]'s Articles
 [Zhu, Haijing]'s Articles
 [Guan, Yafeng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wan, Lingzhong]‘s Articles
 [Zhu, Haijing]‘s Articles
 [Guan, Yafeng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace