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Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine
Wan, Lingzhong1; Zhu, Haijing1; Guan, Yafeng2; Huang, Guangming1
关键词Nanocoating Cellulose Paper Based Microextraction Zirconia Nanospray Mass Spectrometry Ribonucleosides
刊名TALANTA
2017-07-01
DOI10.1016/j.talanta.2017.03.085
169页:209-215
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]LIQUID-CHROMATOGRAPHY ; BREAST-CANCER ; RIBOSYLATED METABOLITES ; SELECTIVE ENRICHMENT ; MODIFIED NUCLEOSIDES ; STATIONARY PHASES ; ZIRCONIA ; TITANIA ; IDENTIFICATION ; NANOPARTICLES
英文摘要A rapid and facile analytical method for quantification of ribonucleosides in human urine was developed by the combination of nanocoating cellulose paper based microextraction and nanoelectrospray ionization-tandem mass spectrometry (nESI-MS/MS). Cellulose paper used for microextraction was modified by nano-precision deposition of uniform ultrathin zirconia gel film using a sol-gel process. Due to the large surface area of the cellulose paper and the strong affinity between zirconia and the cis-diol compounds, the target analytes were selectively extracted from the complex matrix. Thus, the detection sensitivity was greatly improved. Typically, the nanocoating cellulose paper was immersed into the diluted urine for selective extraction of target analytes, then the extracted analytes were subjected to nESI-MS/MS detection. The whole analytical procedure could be completed within 10 min. The method was evaluated by the determination of ribonucleosides (adenosine, cytidine, uridine, guanosine) in urine sample. The signal intensities of the ribonuclesides extracted by the nanocoating cellulose paper were greatly enhanced by 136-459-folds compared with the one of the unmodified cellulose paper based microextraction. The limits of detection (LODs) and the limits of quantification (LOQs) of the four ribonucleosides were in the range of 0.0136-1.258 mu g L-1 and 0.0454-4.194 mu g L-1, respectively. The recoveries of the target nucleosides from spiked human urine were in the range of 75.64-103.49% with the relative standard deviations (RSDs) less than 9.36%. The results demonstrate the potential of the proposed method for rapid and facile determination of endogenous ribonucleosides in urine sample.
语种英语
WOS记录号WOS:000401206700030
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/152314
专题中国科学院大连化学物理研究所
作者单位1.Univ Sci & Technol China, Dept Chem, Sch Chem & Mat Sci, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China
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GB/T 7714
Wan, Lingzhong,Zhu, Haijing,Guan, Yafeng,et al. Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine[J]. TALANTA,2017,169:209-215.
APA Wan, Lingzhong,Zhu, Haijing,Guan, Yafeng,&Huang, Guangming.(2017).Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine.TALANTA,169,209-215.
MLA Wan, Lingzhong,et al."Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine".TALANTA 169(2017):209-215.
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