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Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification
Fang, Shimeng1; Tian, Hongzhu1; Li, Xiancheng2; Jin, Dong1; Li, Xiaojie1; Kong, Jing1; Yang, Chun3; Yang, Xuesong4; Lu, Yao5; Luo, Yong6; Lin, Bingcheng5,6; Niu, Weidong1; Liu, Tingjiao1
Source PublicationPLOS ONE
2017-04-03
DOI10.1371/journal.pone.0175050
Volume12
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordEXTRACELLULAR VESICLES ; MELANOMA EXOSOMES ; CELLS ; EPCAM ; HER2 ; TRASTUZUMAB ; EXPRESSION ; BIOMARKERS ; SYSTEM ; TARGET
AbstractIncreasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes over-express a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis. A significant increase in the EpCAM-positive exosome level in these patients was detected, compared to healthy controls. Furthermore, we quantified circulating HER2-positive exosomes in 19 cases of breast cancer patients for molecular classification. We demonstrated that the exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining. The microfluidic chip might provide a new platform to assist breast cancer diagnosis and molecular classification.
Language英语
WOS IDWOS:000399351000071
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/152329
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dept Urol, Dalian, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dept Nucl Med, Dalian, Peoples R China
4.Dalian Med Univ, Dept Biochem & Mol Biol, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dalian, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
6.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Fang, Shimeng,Tian, Hongzhu,Li, Xiancheng,et al. Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification[J]. PLOS ONE,2017,12.
APA Fang, Shimeng.,Tian, Hongzhu.,Li, Xiancheng.,Jin, Dong.,Li, Xiaojie.,...&Liu, Tingjiao.(2017).Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification.PLOS ONE,12.
MLA Fang, Shimeng,et al."Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification".PLOS ONE 12(2017).
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