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题名: Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification
作者: Fang, Shimeng1;  Tian, Hongzhu1;  Li, Xiancheng2;  Jin, Dong1;  Li, Xiaojie1;  Kong, Jing1;  Yang, Chun3;  Yang, Xuesong4;  Lu, Yao5;  Luo, Yong6;  Lin, Bingcheng5, 6;  Niu, Weidong1;  Liu, Tingjiao1
刊名: PLOS ONE
发表日期: 2017-04-03
DOI: 10.1371/journal.pone.0175050
卷: 12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes over-express a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis. A significant increase in the EpCAM-positive exosome level in these patients was detected, compared to healthy controls. Furthermore, we quantified circulating HER2-positive exosomes in 19 cases of breast cancer patients for molecular classification. We demonstrated that the exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining. The microfluidic chip might provide a new platform to assist breast cancer diagnosis and molecular classification.
关键词[WOS]: EXTRACELLULAR VESICLES ;  MELANOMA EXOSOMES ;  CELLS ;  EPCAM ;  HER2 ;  TRASTUZUMAB ;  EXPRESSION ;  BIOMARKERS ;  SYSTEM ;  TARGET
语种: 英语
WOS记录号: WOS:000399351000071
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/152329
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dept Urol, Dalian, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dept Nucl Med, Dalian, Peoples R China
4.Dalian Med Univ, Dept Biochem & Mol Biol, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dalian, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
6.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian, Peoples R China

Recommended Citation:
Fang, Shimeng,Tian, Hongzhu,Li, Xiancheng,et al. Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification[J]. PLOS ONE,2017,12.
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