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A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance
Wang, Qi1,2; Liu, Zhen1,2; Wang, Keyun1,2; Wang, Yan1,2; Ye, Mingliang1,2
Corresponding AuthorYe, Mingliang(mingliang@dicp.ac.cn)
KeywordMethylproteome Strong cation exchange Immobilized metal ion affinity chromatography High-pH reversed-phase chromatography Lysine methylation identification
Source PublicationANALYTICA CHIMICA ACTA
2019-08-30
ISSN0003-2670
DOI10.1016/j.aca.2019.03.042
Volume1068Pages:111-119
Funding ProjectNational Key R&D Program of China[2016YFA0501402] ; National Key R&D Program of China[2017YFA0505004] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[91753105] ; National Natural Science Foundation of China[21804131] ; National Science Fund of China for Distinguished Young Scholars[21525524]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordNONHISTONE PROTEIN METHYLATION ; MASS-SPECTROMETRY ; ARGININE METHYLATION ; GLOBAL ANALYSIS ; ENRICHMENT ; REGULATOR ; PEPTIDES ; STRATEGY
AbstractArginine/lysine methylation is an important post-translational modification (PTM) involved in DNA repairing, transcriptional regulation, etc. Immunoaffinity enrichment is currently the most widely used methods for the methylproteome analysis. Large-scale analysis of arginine methylation has been realized by using pan-R-methyl antibodies. Unfortunately, pan specific antibodies targeting all three lysine methylation forms are not available. In this study, we presented a novel chromatography-based enrichment method for global methylproteome analysis. The offline multidimensional tandem chromatography combining strong cation exchange (SCX) chromatography, immobilized metal ion affinity chromatography (IMAC) and high-pH reversed-phase chromatography (high-pH RP) was applied in the large-scale analysis of methylproteome. Totally, 860 forms on 765 sites were identified from BEL cells, covering all five arginine/lysine methylation forms. Among them, 27.21% were lysine methylation forms. This technique allows the simultaneous analysis of both arginine and lysine methylation while it has improved performance for the identification of lysine methylation. Therefore, it is a promising strategy for the investigation of biological functions related to methylation. (C) 2019 Elsevier B.V. All rights reserved.
Language英语
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
WOS IDWOS:000466926600013
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165461
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe, Mingliang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qi,Liu, Zhen,Wang, Keyun,et al. A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance[J]. ANALYTICA CHIMICA ACTA,2019,1068:111-119.
APA Wang, Qi,Liu, Zhen,Wang, Keyun,Wang, Yan,&Ye, Mingliang.(2019).A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance.ANALYTICA CHIMICA ACTA,1068,111-119.
MLA Wang, Qi,et al."A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance".ANALYTICA CHIMICA ACTA 1068(2019):111-119.
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