DICP OpenIR
Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry
Liu, Zheyi1; Wang, Ruimin2; Liu, Jing3; Sun, Ruixiang2; Wang, Fangjun1
Corresponding AuthorSun, Ruixiang(rxsun@ict.ac.cn) ; Wang, Fangjun(wangfj@dicp.ac.cn)
Keywordmass spectrometry chemical isotope labeling intact protein quantification pTOP highest isotopic peak
Source PublicationJOURNAL OF PROTEOME RESEARCH
2019-05-01
ISSN1535-3893
DOI10.1021/acs.jproteome.9b00071
Volume18Issue:5Pages:2185-2194
Funding ProjectNational Key R&D Program of China[2016YFF0200504] ; National Natural Science Foundation of China[21675152] ; National Natural Science Foundation of China[91853101] ; National Natural Science Foundation of China[31670837] ; DICP[ZZBS201603]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordTOP-DOWN PROTEOMICS ; FORMALDEHYDE-INDUCED MODIFICATIONS ; CELL-CULTURE ; AMINO-ACIDS ; SACCHAROMYCES-CEREVISIAE ; QUANTITATIVE PROTEOMICS ; REVERSED-PHASE ; WINE YEAST ; IDENTIFICATION ; EXPRESSION
AbstractAlthough thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved in the important biological processes such as stress response.
Language英语
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP
WOS IDWOS:000467317700023
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165471
Collection中国科学院大连化学物理研究所
Corresponding AuthorSun, Ruixiang; Wang, Fangjun
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
3.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zheyi,Wang, Ruimin,Liu, Jing,et al. Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(5):2185-2194.
APA Liu, Zheyi,Wang, Ruimin,Liu, Jing,Sun, Ruixiang,&Wang, Fangjun.(2019).Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,18(5),2185-2194.
MLA Liu, Zheyi,et al."Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 18.5(2019):2185-2194.
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