DICP OpenIR
Enhanced acetic acid stress tolerance and ethanol production in Saccharomyces cerevisiae by modulating expression of the de novo purine biosynthesis genes
Zhang, Ming-Ming1; Xiong, Liang3; Tang, Ya-Jie2,5; Mehmood, Muhammad Aamer1,6; Zhao, Zongbao Kent4; Bai, Feng-Wu1; Zhao, Xin-Qing1
Corresponding AuthorZhao, Xin-Qing(xqzhao@sjtu.edu.cn)
KeywordSaccharomyces cerevisiae Yeast stress tolerance de novo purine biosynthesis ADE17 Global amino acid profiles
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
2019-05-10
ISSN1754-6834
DOI10.1186/s13068-019-1456-1
Volume12Pages:13
Funding ProjectNational Natural Science Foundation of China[21536006] ; National Natural Science Foundation of China[51561145014] ; Chinese Postdoctoral Science Foundation[19Z102060011]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS KeywordFERMENTATION PERFORMANCE ; YEAST ; ZINC ; OVEREXPRESSION ; DELETION ; METABOLISM ; XYLOSE ; SUPPLEMENTATION ; NUCLEOTIDE ; INHIBITORS
AbstractBackgroundYeast strains that are tolerant to multiple environmental stresses are highly desired for various industrial applications. Despite great efforts in identifying key genes involved in stress tolerance of budding yeast Saccharomyces cerevisiae, the effects of de novo purine biosynthesis genes on yeast stress tolerance are still not well explored. Our previous studies showed that zinc sulfate addition improved yeast acetic acid tolerance, and key genes involved in yeast stress tolerance were further investigated in this study.ResultsThree genes involved in de novo purine biosynthesis, namely, ADE1, ADE13, and ADE17, showed significantly increased transcription levels by zinc sulfate supplementation under acetic acid stress, and overexpression of these genes in S. cerevisiae BY4741 enhanced cell growth under various stress conditions. Meanwhile, ethanol productivity was also improved by overexpression of the three ADE genes under stress conditions, among which the highest improvement attained 158.39% by ADE17 overexpression in the presence of inhibitor mixtures derived from lignocellulosic biomass. Elevated levels of adenine-nucleotide pool AXP ([ATP]+[ADP]+[AMP]) and ATP content were observed by overexpression of ADE17, both under control condition and under acetic acid stress, and is consistent with the better growth of the recombinant yeast strain. The global intracellular amino acid profiles were also changed by overexpression of the ADE genes. Among the changed amino acids, significant increase of the stress protectant -aminobutyric acid (GABA) was revealed by overexpression of the ADE genes under acetic acid stress, suggesting that overexpression of the ADE genes exerts control on both purine biosynthesis and amino acid biosynthesis to protect yeast cells against the stress.ConclusionWe proved that the de novopurine biosynthesis genes are useful targets for metabolic engineering of yeast stress tolerance. The engineered strains developed in this study with improved tolerance against multiple inhibitors can be employed for efficient lignocellulosic biorefinery to produce biofuels and biochemicals.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Postdoctoral Science Foundation ; Chinese Postdoctoral Science Foundation
WOS IDWOS:000467549800001
PublisherBMC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165495
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao, Xin-Qing
Affiliation1.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
2.Hubei Univ Technol, Key Lab Fermentat Engn, Minist Educ, Hubei Prov Cooperat Innovat Ctr Ind Fermentat,Hub, Wuhan 430068, Hubei, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
4.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
5.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China
6.Univ Faisalabad, Dept Bioinformat & Biotechnol, Govt Coll, Faisalabad 38000, Pakistan
Recommended Citation
GB/T 7714
Zhang, Ming-Ming,Xiong, Liang,Tang, Ya-Jie,et al. Enhanced acetic acid stress tolerance and ethanol production in Saccharomyces cerevisiae by modulating expression of the de novo purine biosynthesis genes[J]. BIOTECHNOLOGY FOR BIOFUELS,2019,12:13.
APA Zhang, Ming-Ming.,Xiong, Liang.,Tang, Ya-Jie.,Mehmood, Muhammad Aamer.,Zhao, Zongbao Kent.,...&Zhao, Xin-Qing.(2019).Enhanced acetic acid stress tolerance and ethanol production in Saccharomyces cerevisiae by modulating expression of the de novo purine biosynthesis genes.BIOTECHNOLOGY FOR BIOFUELS,12,13.
MLA Zhang, Ming-Ming,et al."Enhanced acetic acid stress tolerance and ethanol production in Saccharomyces cerevisiae by modulating expression of the de novo purine biosynthesis genes".BIOTECHNOLOGY FOR BIOFUELS 12(2019):13.
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