DICP OpenIR
NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction
Wang, Jianshu1,2; Chen, Jiyun3; Wu, Guifen1,2; Zhang, Hongling2,4; Du, Xian5; Chen, Suli1,2; Zhang, Li1,2; Wang, Ke1,2; Fan, Jing1,2; Gao, Shuaixin6; Wu, Xudong7; Zhang, Shouxiang3; Kuai, Bin1,2; Zhao, Peng3; Chi, Binkai1,2; Wang, Lantian1,2; Li, Guohui7; Wong, Catherine C. L.6,8; Zhou, Yu5; Li, Jinsong2,4; Yun, Caihong3; Cheng, Hong1,2
Corresponding AuthorZhou, Yu(yu.zhou@whu.edu.cn) ; Li, Jinsong(jsli@sibcb.ac.cn) ; Yun, Caihong(yunch@hsc.pku.edu.cn) ; Cheng, Hong(hcheng@sibcb.ac.cn)
KeywordNRDE2 the nuclear exosome MTR4 recruitment mRNA export
Source PublicationGENES & DEVELOPMENT
2019-05-01
ISSN0890-9369
DOI10.1101/gad.322602.118
Volume33Issue:9-10Pages:536-549
Funding ProjectNational Natural Science Foundation of China[31770880] ; National Natural Science Foundation of China[31570822] ; National Natural Science Foundation of China[31800686] ; National Key R&D Program of China[2017YFA0504400] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB19000000] ; National Science Foundation of China[31270769]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China
WOS SubjectCell Biology ; Developmental Biology ; Genetics & Heredity
WOS Research AreaCell Biology ; Developmental Biology ; Genetics & Heredity
WOS KeywordCRYO-EM STRUCTURE ; NUCLEAR EXOSOME ; CRYSTAL-STRUCTURE ; QUALITY CONTROL ; MESSENGER-RNAS ; TREX COMPLEX ; ARCH DOMAIN ; HELICASE ; DEGRADATION ; PROTEIN
AbstractThe exosome functions in the degradation of diverse RNA species, yet how it is negatively regulated remains largely unknown. Here, we show that NRDE2 forms a 1:1 complex with MTR4, a nuclear exosome cofactor critical for exosome recruitment, via a conserved MTR4-interacting domain (MID). Unexpectedly, NRDE2 mainly localizes in nuclear speckles, where it inhibits MTR4 recruitment and RNA degradation, and thereby ensures efficient mRNA nuclear export. Structural and biochemical data revealed that NRDE2 interacts with MTR4's key residues, locks MTR4 in a closed conformation, and inhibits MTR4 interaction with the exosome as well as proteins important for MTR4 recruitment, such as the cap-binding complex (CBC) and ZFC3H1. Functionally, MID deletion results in the loss of self-renewal of mouse embryonic stem cells. Together, our data pinpoint NRDE2 as a nuclear exosome negative regulator that ensures mRNA stability and nuclear export.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science Foundation of China ; National Science Foundation of China
WOS IDWOS:000466358800006
PublisherCOLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165503
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhou, Yu; Li, Jinsong; Yun, Caihong; Cheng, Hong
Affiliation1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,State Key Lab Mol, Shanghai 200031, Peoples R China
2.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Shanghai Key Lab, Shanghai 200031, Peoples R China
3.Peking Univ Hlth Sci Ctr, Beijing Key Lab Tumor Syst Biol, Sch Basic Med Sci, Dept Biophys, Beijing 100191, Peoples R China
4.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,State Key Lab Cel, Shanghai 200031, Peoples R China
5.Wuhan Univ, Coll Life Sci, Hubei Key Lab Cell Homeostasis, Wuhan 430072, Hubei, Peoples R China
6.Peking Univ Hlth Sci Ctr, Ctr Precis Med Multiom Res, Beijing 100191, Peoples R China
7.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
8.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jianshu,Chen, Jiyun,Wu, Guifen,et al. NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction[J]. GENES & DEVELOPMENT,2019,33(9-10):536-549.
APA Wang, Jianshu.,Chen, Jiyun.,Wu, Guifen.,Zhang, Hongling.,Du, Xian.,...&Cheng, Hong.(2019).NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.GENES & DEVELOPMENT,33(9-10),536-549.
MLA Wang, Jianshu,et al."NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction".GENES & DEVELOPMENT 33.9-10(2019):536-549.
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