DICP OpenIR
Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites
Zeng, Jun1,6; Wang, Zhichao1,6; Huang, Xin2; Eckstein, Sabine S.3,4,5; Lin, Xiaohui2; Piao, Hailong1; Weigert, Cora3,4,5; Yin, Peiyuan1; Lehmann, Rainer3,4,5; Xu, Guowang1
Corresponding AuthorLehmann, Rainer(rainer.lehmann@med.uni-tuebingen.de) ; Xu, Guowang(xugw@dicp.ac.cn)
Keywordcapillary electrophoresis isotopologues mass spectrometry metabolism metabolomics
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
2019-04-11
ISSN0947-6539
DOI10.1002/chem.201900539
Volume25Issue:21Pages:5427-5432
Funding ProjectNational Key R&D Program of China[2017YFC0906900] ; National Natural Science Foundation of China[21707042] ; National Natural Science Foundation of China[81472374] ; National Natural Science Foundation of China[21375011] ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG)[GZ 753] ; Sino-German Center for Research Promotion (DFG)[LE 1391/1-1]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG)
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordHEPATOCELLULAR-CARCINOMA ; PROSTATE-CANCER ; SERINE ; BIOMARKERS
AbstractMass spectrometry (MS) driven metabolomics is a frequently used tool in various areas of life sciences; however, the analysis of polar metabolites is less commonly included. In general, metabolomic analyses lead to the detection of the total amount of all covered metabolites. This is currently a major limitation with respect to metabolites showing high turnover rates, but no changes in their concentration. Such metabolites and pathways could be crucial metabolic nodes (e.g., potential drug targets in cancer metabolism). A stable-isotope tracing capillary electrophoresis-mass spectrometry (CE-MS) metabolomic approach was developed to cover both polar metabolites and isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multidimensional data. The practicability is demonstrated analyzing [U-C-13]-glucose exposed prostate cancer and non-cancer cells. This CE-MS-driven analytical strategy complements polar metabolite profiles through isotopologue labeling patterns, thereby improving not only the metabolomic coverage, but also the understanding of metabolism.
Language英语
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG)
WOS IDWOS:000466018300009
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165523
Collection中国科学院大连化学物理研究所
Corresponding AuthorLehmann, Rainer; Xu, Guowang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Dept Comp Sci & Engn, Dalian 116024, Peoples R China
3.Univ Hosp Tubingen, Dept Diagnost Lab Med, Inst Clin Chem & Pathobiochem, D-72076 Tubingen, Germany
4.Univ Hosp Tubingen, Inst Diabet Res & Metab Dis, German Ctr Diabet Res DZD, Clin Chem Lab,Core Facil, D-72076 Tubingen, Germany
5.German Ctr Diabet Res DZD, Tubingen, Germany
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Jun,Wang, Zhichao,Huang, Xin,et al. Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites[J]. CHEMISTRY-A EUROPEAN JOURNAL,2019,25(21):5427-5432.
APA Zeng, Jun.,Wang, Zhichao.,Huang, Xin.,Eckstein, Sabine S..,Lin, Xiaohui.,...&Xu, Guowang.(2019).Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites.CHEMISTRY-A EUROPEAN JOURNAL,25(21),5427-5432.
MLA Zeng, Jun,et al."Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites".CHEMISTRY-A EUROPEAN JOURNAL 25.21(2019):5427-5432.
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