DICP OpenIR
Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS
Xu, Yanqiu1; Sun, Luping1; Wang, Xin1; Zhu, Shuyun1; You, Jinmao1; Zhao, Xian-En1; Bai, Yu2; Liu, Huwei2
Corresponding AuthorZhao, Xian-En(xianenzhao@163.com)
KeywordNeurosteroids Isotope-coded derivatization Magnetic separation Triple quadrupole mass spectrometry In vivo microdialysis technique Parkinson's disease
Source PublicationTALANTA
2019-07-01
ISSN0039-9140
DOI10.1016/j.talanta.2019.02.011
Volume199Pages:97-106
Funding ProjectNational Natural Science Foundation of China[21775088] ; National Natural Science Foundation of China[21527809] ; National Natural Science Foundation of China[21575007] ; National Natural Science Foundation of China[21775008] ; National Natural Science Foundation of China[21405094] ; National Natural Science Foundation of China[81303179] ; Open Research Fund of Beijing National Laboratory for Molecular Sciences[BNLMS20160147] ; Foundation of Qufu Normal University[BSQD2012019] ; Foundation of Qufu Normal University[BSQD2012023]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; ENVIRONMENTAL WATER SAMPLES ; GREEN SYNTHESIS STRATEGY ; MASS-SPECTROMETRY ; QUANTITATIVE-ANALYSIS ; BIOLOGICAL SAMPLES ; CARBOXYLIC-ACIDS ; GRAPHENE OXIDE ; RAT-BRAIN ; NEUROTRANSMITTERS
AbstractIn this work, a novel strategy of stable isotope labeling derivatization (SILD) combined with magnetic dispersive solid-phase extraction (MDSPE), has been proposed for simultaneous monitoring of neurosteroids changes linked to Parkinson's disease (PD) by in vivo microdialysis. The developed method was based on ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection using multiple-reaction monitoring (MRM) mode. In this study, a new pair of stable isotope labeling reagents d(0)-/d(3)-3-N-methyl-2'-carboxyl Rhodamine 6G (d(0)-/d(3)-MCR6G), were designed and synthesized for derivatizing neurosteroids in rat blood microdialysates and neurosteroid standards, respectively. d(3)-MCR6G labeled neurosteroids standards were used as internal standard for the following pretreatment and UHPLC-MS/MS quantification to minimize the deviations caused by complex matrix and ion suppression effects in mass spectrometry analysis. Under the optimized derivatization and extraction conditions, good linearities of eight neurosteroids were obtained with correlation coefficients R-2 values > 0.98. The limits of detection (LODs) and quantitation (LOQs) ranged from 0.06 to 0.12 pg/mL and 0.30-0.40 pg/mL, respectively. Taken together, the established method exhibited high sensitivity and selectivity, excellent accuracy, convenience and high efficiency. It was applied for the simultaneous and dynamic measurement of multiple neurosteroids in normal and PD rat blood microdialysates. This method would be expected to be potentially useful for the monitoring and drug treatment of PD and related neurological disorders in the future.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University
WOS IDWOS:000465050000014
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165555
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao, Xian-En
Affiliation1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China
2.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Xu, Yanqiu,Sun, Luping,Wang, Xin,et al. Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS[J]. TALANTA,2019,199:97-106.
APA Xu, Yanqiu.,Sun, Luping.,Wang, Xin.,Zhu, Shuyun.,You, Jinmao.,...&Liu, Huwei.(2019).Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS.TALANTA,199,97-106.
MLA Xu, Yanqiu,et al."Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS".TALANTA 199(2019):97-106.
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