DICP OpenIR
Core Fucosylation of Maternal Milk N-Glycan Evokes B Cell Activation by Selectively Promoting the L-Fucose Metabolism of Gut Bifidobacterium spp. and Lactobacillus spp.
Li, Ming1; Bai, Yaqiang1; Zhou, Jiaorui1; Huang, Wei2; Yan, Jingyu3; Tao, Jia4; Fan, Qingjie1; Liu, Yang5; Mei, Di5; Yan, Qiulong1; Yuan, Jieli1; Malard, Patrice6; Wang, Zhongfu7; Gu, Jianguo8; Tanigchi, Naoyuki9; Li, Wenzhe1
Corresponding AuthorLi, Ming(vivianmarat@163.com) ; Li, Wenzhe(liwenzhe@dlmedu.edu.cn)
KeywordB cells Bifidobacterium Lactobacillus core fucosylation infants milk N-glycan
Source PublicationMBIO
2019-03-01
ISSN2150-7511
DOI10.1128/mBio.00128-19
Volume10Issue:2Pages:19
Funding ProjectNational Natural Science Foundation of China[31570797] ; National Natural Science Foundation of China[31870797] ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care[2018BINCMCF39] ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care[2016BINCMCF1102] ; Research Foundation from the Department of Education, Liaoning Province, China[L2016003] ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China
WOS SubjectMicrobiology
WOS Research AreaMicrobiology
WOS KeywordMICROBIOTA COMPOSITION ; LIQUID-CHROMATOGRAPHY ; OLIGOSACCHARIDES ; GROWTH ; RECEPTOR ; CASEI ; IDENTIFICATION ; GLYCOPROTEINS ; LECTIN ; GLYCOSYLATION
AbstractThe maternal milk glycobiome is crucial for shaping the gut microbiota of infants. Although high core fucosylation catalyzed by fucosyltransferase 8 (Fut8) is a general feature of human milk glycoproteins, its role in the formation of a healthy microbiota has not been evaluated. In this study, we found that the core-fucosylated N-glycans in milk of Chinese mothers selectively promoted the colonization of specific gut microbial groups, such as Bifidobacterium spp. and Lactobacillus spp. in their breast-fed infants during lactation. Compared with Fut8(+/-) (WT) mouse-fed neonates, the offspring fed by Fut8(+/+) maternal mice had a distinct gut microbial profile, which was featured by a significant reduction of Lactobacillus spp., Bacteroides spp., and Bifidobacterium spp. and increased abundance of members of the Lachno-spiraceae NK4A136 group and Akkermansia spp. Moreover, these offspring mice showed a lower proportion of splenic CD19(+) CD69(+) B lymphocytes and attenuated humoral immune responses upon ovalbumin (OVA) immunization. In vitro studies demonstrated that the chemically synthesized core-fucosylated oligosaccharides possessed the ability to promote the growth of tested Bifidobacterium and Lactobacillus strains in minimal medium. The resulting L-fucose metabolites, lactate and 1,2-propanediol, could promote the activation of B cells via the B cell receptor (BCR)-mediated signaling pathway. IMPORTANCE This study provides novel evidence for the critical role of maternal milk protein glycosylation in shaping early-life gut microbiota and promoting B cell activation of neonates. The special core-fucosylated oligosaccharides might be promising prebiotics for the personalized nutrition of infants.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Research Foundation from the Department of Education, Liaoning Province, China ; Research Foundation from the Department of Education, Liaoning Province, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China ; Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China
WOS IDWOS:000465077600055
PublisherAMER SOC MICROBIOLOGY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165587
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Ming; Li, Wenzhe
Affiliation1.Dalian Med Univ, Coll Basic Med Sci, Dalian, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
4.Jinzhou Med Univ, Affiliated Hosp 1, Dept Gynaecol, Jinzhou, Peoples R China
5.Huludao Ctr Hosp, Clin Lab, Huludao, Peoples R China
6.BINC, Guangzhou, Guangdong, Peoples R China
7.Northwest Univ, Life Sci Coll, Educ Minist, Key Lab Resource Biol & Biotechnol Western China, Xian, Shaanxi, Peoples R China
8.Tohoku Med & Pharmaceut Univ, Inst Mol Biomembrane & Glycobiol, Div Regulatory Glycobiol, Sendai, Miyagi, Japan
9.Osaka Int Canc Inst, Dept Glyco Oncol, Osaka, Japan
Recommended Citation
GB/T 7714
Li, Ming,Bai, Yaqiang,Zhou, Jiaorui,et al. Core Fucosylation of Maternal Milk N-Glycan Evokes B Cell Activation by Selectively Promoting the L-Fucose Metabolism of Gut Bifidobacterium spp. and Lactobacillus spp.[J]. MBIO,2019,10(2):19.
APA Li, Ming.,Bai, Yaqiang.,Zhou, Jiaorui.,Huang, Wei.,Yan, Jingyu.,...&Li, Wenzhe.(2019).Core Fucosylation of Maternal Milk N-Glycan Evokes B Cell Activation by Selectively Promoting the L-Fucose Metabolism of Gut Bifidobacterium spp. and Lactobacillus spp..MBIO,10(2),19.
MLA Li, Ming,et al."Core Fucosylation of Maternal Milk N-Glycan Evokes B Cell Activation by Selectively Promoting the L-Fucose Metabolism of Gut Bifidobacterium spp. and Lactobacillus spp.".MBIO 10.2(2019):19.
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