DICP OpenIR
High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework
Peng, Jiaxi1,2; Hu, Yechen1,2; Zhang, Hongyan1; Wan, Lihong1; Wang, Li1; Liang, Zhen1; Zhang, Lihua1; Wei, Ren'an1
Corresponding AuthorZhang, Lihua(lihuazhang@dicp.ac.cn) ; Wei, Ren'an(wurenan@dicp.ac.cn)
Source PublicationANALYTICAL CHEMISTRY
2019-04-02
ISSN0003-2700
DOI10.1021/acs.analchem.9b00542
Volume91Issue:7Pages:4852-4859
Funding ProjectNational Natural Science Foundation of China[21675156] ; National Natural Science Foundation of China[21375125] ; National Natural Science Foundation of China[21725506] ; National Natural Science Foundation of China[91543201] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201503] ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China[2017YFA0505003] ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics[DICP TMSR201601]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSITE-SPECIFIC CHARACTERIZATION ; EFFICIENT ENRICHMENT ; SERUM PEPTIDES ; GLYCOSYLATION ; EXTRACTION ; MECHANISMS ; REVEALS
AbstractGlycopeptidome profiling provides large-scale information about the glycosylation level of endogenous peptides, reflecting the dynamic processes of disease occurrences and developments. However, endogenous glycopeptides are usually submerged in complex fluids containing a wide variety of interference molecules, such as high concentration proteins, nonglycopeptides, and salts, which confounds attempts to identify glycopeptidome. Here, a dual hydrophilic metal organic framework is developed to selectively capture endogenous glycopeptides in complex biological fluid. The hydrophilic matrix material provides specific selectivity toward glycopeptides, while the deliberate surface regulation using hydrophilic species enhances its interaction with glycopeptides. This hydrophilic probe presents an extremely high performance in anti-interfering enrichment of glycopeptides from mimic complex samples, even when the molar ratio of immunoglobulin G versus bovine serum albumin was up to about 1:5000. More excitingly, in the practical application of glycopeptidome analysis, a total of 380 endogenous N-glycopeptides with 180 unique N-glycopeptide sites were identified from human plasma. This strategy is expected to broaden the application of dual-hydrophilic MOF-based materials, especially in dealing with the challenges of extremely complex biological samples.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
WOS IDWOS:000463683300078
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165631
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua; Wei, Ren'an
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Peng, Jiaxi,Hu, Yechen,Zhang, Hongyan,et al. High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework[J]. ANALYTICAL CHEMISTRY,2019,91(7):4852-4859.
APA Peng, Jiaxi.,Hu, Yechen.,Zhang, Hongyan.,Wan, Lihong.,Wang, Li.,...&Wei, Ren'an.(2019).High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework.ANALYTICAL CHEMISTRY,91(7),4852-4859.
MLA Peng, Jiaxi,et al."High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework".ANALYTICAL CHEMISTRY 91.7(2019):4852-4859.
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