DICP OpenIR
Multiplexed profiling of single-cell extracellular vesicles secretion
Ji, Yahui1,2; Qi, Dongyuan3; Li, Linmei1; Su, Haoran1,2; Li, Xiaojie2; Luo, Yong4; Sun, Bo5; Zhang, Fuyin5; Lin, Bingcheng1; Liu, Tingjiao2; Lu, Yao1
Corresponding AuthorLiu, Tingjiao(tingjiao@dmu.edu.cn) ; Lu, Yao(luyao@dicp.ac.cn)
Keywordsingle-cell analysis extracellular vesicle cellular heterogeneity antibody barcodes
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2019-03-26
ISSN0027-8424
DOI10.1073/pnas.1814348116
Volume116Issue:13Pages:5979-5984
Funding ProjectNational Natural Science Foundation of China[21874133] ; National Natural Science Foundation of China[21605143] ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant[2018217] ; Dalian Institute of Chemical Physics Grant[SZ201601]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordCD63 TETRASPANIN ; EXOSOME SECRETION ; HETEROGENEITY ; REVEALS ; EXPRESSION ; MICROVESICLES ; CARCINOMA ; CYTOKINE ; RELEASE
AbstractExtracellular vesicles (EVs) are important intercellular mediators regulating health and diseases. Conventional methods for EV surface marker profiling, which was based on population measurements, masked the cell-to-cell heterogeneity in the quantity and phenotypes of EV secretion. Herein, by using spatially patterned antibody barcodes, we realized multiplexed profiling of single-cell EV secretion from more than 1,000 single cells simultaneously. Applying this platform to profile human oral squamous cell carcinoma (OSCC) cell lines led to a deep understanding of previously undifferentiated single-cell heterogeneity underlying EV secretion. Notably, we observed that the decrement of certain EV phenotypes (e.g., (CD63+) V) was associated with the invasive feature of both OSCC cell lines and primary OSCC cells. We also realized multiplexed detection of EV secretion and cytokines secretion simultaneously from the same single cells to investigate the multidimensional spectrum of cellular communications, from which we resolved tiered functional subgroups with distinct secretion profiles by visualized clustering and principal component analysis. In particular, we found that different cell subgroups dominated EV secretion and cytokine secretion. The technology introduced here enables a comprehensive evaluation of EV secretion heterogeneity at single-cell level, which may become an indispensable tool to complement current single-cell analysis and EV research.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant
WOS IDWOS:000462382800029
PublisherNATL ACAD SCIENCES
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165703
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Tingjiao; Lu, Yao
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dalian 116011, Peoples R China
4.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
5.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
Recommended Citation
GB/T 7714
Ji, Yahui,Qi, Dongyuan,Li, Linmei,et al. Multiplexed profiling of single-cell extracellular vesicles secretion[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(13):5979-5984.
APA Ji, Yahui.,Qi, Dongyuan.,Li, Linmei.,Su, Haoran.,Li, Xiaojie.,...&Lu, Yao.(2019).Multiplexed profiling of single-cell extracellular vesicles secretion.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(13),5979-5984.
MLA Ji, Yahui,et al."Multiplexed profiling of single-cell extracellular vesicles secretion".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.13(2019):5979-5984.
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