DICP OpenIR
A New Searching Strategy for the Identification of O-Linked Glycopeptides
Mao, Jiawei1,2; You, Xin1,2; Qin, Hongqiang1,2; Wang, Chenye3; Wang, Liming3; Ye, Mingliang1,2
Corresponding AuthorYe, Mingliang(mingliang@dicp.ac.cn)
Source PublicationANALYTICAL CHEMISTRY
2019-03-19
ISSN0003-2700
DOI10.1021/acs.analchem.8b04184
Volume91Issue:6Pages:3852-3859
Funding ProjectChina State Key Basic Research Program[2018YFC0910302] ; China State Key Basic Research Program[2016YFA0501402] ; China State Key Basic Research Program[2017YFA0505003] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[81430072] ; National Natural Science Foundation of China[21775146] ; DICP, CAS[DICP TMSR201601] ; National Science Fund of China for Distinguished Young Scholars[21525524]
Funding OrganizationChina State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordINTACT N-GLYCOPEPTIDES ; GLYCOSYLATION ; GLYCOPROTEOME ; PROTEOMICS ; SPECTRA ; GLYCANS ; ROLES
AbstractFor the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching. However, this approach is often not applicable to identify intact mucin-type O-glycopeptides due to the high microheterogeneity of the glycosylation. Because there is virtually no carbohydrate-related tag on the peptide fragments after the O-glycopeptides are dissociated in HCD, we find it is unnecessary to set the variable mass tags on the Ser/Thr residues to identify the peptide sequences. In this study, we present a novel approach, termed as O-Search, for the interpretation of O-glycopeptide HCD spectra. Instead of setting the variable mass tags on the Ser/Thr residues, we set variable mass tags on the peptide level. The precursor mass of the MS/MS spectrum was deducted by every possible summed mass of O-glycan combinations on at most three S/T residues. All the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications. It was found that this method had much decreased search space and had excellent sensitivity in the identification of O-glycopeptides. Compared with the conventional searching approach, O-Search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively. It was demonstrated that O-Search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of O-glycosylation.
Language英语
Funding OrganizationChina State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program ; China State Key Basic Research Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP, CAS ; DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
WOS IDWOS:000462098300015
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165733
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe, Mingliang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Hosp 2, Dept Surg, Div Hepatobiliary & Pancreat Surg, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Mao, Jiawei,You, Xin,Qin, Hongqiang,et al. A New Searching Strategy for the Identification of O-Linked Glycopeptides[J]. ANALYTICAL CHEMISTRY,2019,91(6):3852-3859.
APA Mao, Jiawei,You, Xin,Qin, Hongqiang,Wang, Chenye,Wang, Liming,&Ye, Mingliang.(2019).A New Searching Strategy for the Identification of O-Linked Glycopeptides.ANALYTICAL CHEMISTRY,91(6),3852-3859.
MLA Mao, Jiawei,et al."A New Searching Strategy for the Identification of O-Linked Glycopeptides".ANALYTICAL CHEMISTRY 91.6(2019):3852-3859.
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