DICP OpenIR
Simultaneous Quantification of Ten Oxysterols Based on LC-MS/MS and its Application in Atherosclerosis Human Serum Samples
Chen, Lishan1; Xiu, Rui2; Wang, Huan1; Wang, Longxing3; Wu, Guanmin1; Liang, Jian1; Han, Xiaofei1
Corresponding AuthorHan, Xiaofei(helarpmi1640@126.com)
KeywordLC-MS MS Oxysterols Human serum Derivatization Atherosclerosis
Source PublicationCHROMATOGRAPHIA
2019-02-01
ISSN0009-5893
DOI10.1007/s10337-018-3654-6
Volume82Issue:2Pages:553-564
Funding ProjectNational Natural Science Foundation of China[81400337] ; National Natural Science Foundation of China[81673417]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; LIVER X RECEPTOR ; LIPOPROTEIN ; OXIDATION ; PRODUCTS ; STEROLS ; PLASMA ; DERIVATIVES ; EXTRACTION ; DISEASE
AbstractA highly sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS/MS) method has been developed and validated for the identification and relative quantification of ten oxysterols in human serum. After alkaline hydrolysis and derivatization, oxysterols in 50 mu L human serum were extracted and converted to corresponding picolinyl ester derivatives. The freshly prepared samples were analyzed by LC-MS/MS in high efficiency; and these ten oxysterols were separated within 11min. The method enabled the determination of standards with limits of detection between 0.0048 and 0.14ng/mL. The variances between sample preparations and measurements by this method were 3.28-8.47% and 2.01-7.26%, respectively. Recoveries of the oxysterols and cholesterol ranged from 90.49 to 121.01%, with the mean recovery of 102.63%. The rapid LC-MS/MS method enables relative quantification of oxysterols in human serum under high-throughput conditions. Furthermore, the established method has been applied to analyze the content of ten oxysterols in serum of healthy people and atherosclerotic. The results showed that the levels of 25-hydroxycholesterol and cholestane-3 ss,5,6 ss-triol in patients were significantly different from healthy people. This current method might provide the opportunity for clinical application. [GRAPHICS] .
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000460411700007
PublisherSPRINGER HEIDELBERG
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165785
Collection中国科学院大连化学物理研究所
Corresponding AuthorHan, Xiaofei
Affiliation1.Dalian Univ, Key Lab Carbohydrate & Lipid Metab Res, Coll Life Sci & Technol, 10 Xuefu Ave, Dalian 116622, Liaoning, Peoples R China
2.Dalian Sanat, Dept Pharm, Dalian 116013, Liaoning, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Chen, Lishan,Xiu, Rui,Wang, Huan,et al. Simultaneous Quantification of Ten Oxysterols Based on LC-MS/MS and its Application in Atherosclerosis Human Serum Samples[J]. CHROMATOGRAPHIA,2019,82(2):553-564.
APA Chen, Lishan.,Xiu, Rui.,Wang, Huan.,Wang, Longxing.,Wu, Guanmin.,...&Han, Xiaofei.(2019).Simultaneous Quantification of Ten Oxysterols Based on LC-MS/MS and its Application in Atherosclerosis Human Serum Samples.CHROMATOGRAPHIA,82(2),553-564.
MLA Chen, Lishan,et al."Simultaneous Quantification of Ten Oxysterols Based on LC-MS/MS and its Application in Atherosclerosis Human Serum Samples".CHROMATOGRAPHIA 82.2(2019):553-564.
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