DICP OpenIR
Milk digestion peptidomics: Tracking caseinophosphopeptides in simulated gastrointestinal digestion
Yu, Yang1; Qi, Yanxia2; Jin, Yan1
Corresponding AuthorQi, Yanxia(qiyanxia@dlou.edu.cn) ; Jin, Yan(yanjin@dicp.ac.cn)
KeywordCaseinophosphopeptides Milk Simulated gastrointestinal digestion
Source PublicationFOOD BIOSCIENCE
2019-04-01
ISSN2212-4292
DOI10.1016/j.fbio.2019.01.019
Volume28Pages:143-150
Funding ProjectNational Natural Science Foundation of China[21235006] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[21525524] ; National Natural Science Foundation of China[31601538]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectFood Science & Technology
WOS Research AreaFood Science & Technology
WOS KeywordCASEIN PHOSPHOPEPTIDES ; INHIBITORY PEPTIDES ; BOVINE CASEIN ; BETA-CASEIN ; IDENTIFICATION ; ENRICHMENT ; IRON ; HYDROLYSIS ; PROTEINS ; BINDING
AbstractThe dynamics of caseinophosphopeptides (CPP) in simulated gastrointestinal digestion was studied. CPP in the undigested milk and in vitro simulated gastrointestinal hydrolysates were enriched using immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) beads and measured using liquid chromatography-tandem mass spectrometry. There were 62, 80 and 38 CPP identified from undigested milk, simulated gastric and pancreatic hydrolysates, respectively. In total, 142 unique CPP and 27 casein phosphorylation sites were identified. Only 5 of 62 endogenous CPP in undigested milk survived in the simulated gastrointestinal hydrolysates and these 5 CPP were semi-quantified. Most of the CPP in undigested milk came from beta-casein. Further, 13 CPP with phosphothreonine were found in simulated gastrointestinal hydrolysates. Seven CPP with the conserved sequence SpSpSpEE were identified from simulated gastrointestinal hydrolysates. Only 2 of the 6 CPP with the conserved sequence SpSpSpEE in undigested milk survived in the simulated gastric digestion, and all of them were digested by pancreatin during simulated pancreatic digestion. The results showed that most of the CPP were released from large endogenous CPP or caseins in simulated gastrointestinal hydrolysates. However, most of them were degraded after simulated pancreatic digestion while the content of some persistent CPP increased at the same time.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000460408800020
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165815
Collection中国科学院大连化学物理研究所
Corresponding AuthorQi, Yanxia; Jin, Yan
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
2.Dalian Ocean Univ, 52 Heishijiao St, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Yu, Yang,Qi, Yanxia,Jin, Yan. Milk digestion peptidomics: Tracking caseinophosphopeptides in simulated gastrointestinal digestion[J]. FOOD BIOSCIENCE,2019,28:143-150.
APA Yu, Yang,Qi, Yanxia,&Jin, Yan.(2019).Milk digestion peptidomics: Tracking caseinophosphopeptides in simulated gastrointestinal digestion.FOOD BIOSCIENCE,28,143-150.
MLA Yu, Yang,et al."Milk digestion peptidomics: Tracking caseinophosphopeptides in simulated gastrointestinal digestion".FOOD BIOSCIENCE 28(2019):143-150.
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