DICP OpenIR
Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides
Zhu, Xian-Yu1,2; Zhao, Yong1; Zhang, Huai-Dong1,3; Wang, Wen-Xia1; Cong, Hai-Hua2; Yin, Heng1
Corresponding AuthorYin, Heng(yinheng@dicp.ac.cn)
Keywordchitin deacetylase deacetylation patterns chitooligosaccharides separating detecting
Source PublicationMARINE DRUGS
2019-02-01
ISSN1660-3397
DOI10.3390/md17020074
Volume17Issue:2Pages:15
Funding ProjectNational Natural Science Foundation of China[31670803] ; National Natural Science Foundation of China[31770847] ; National Key Research and Development Project of China[2017YFD0200902] ; Dalian city Innovative Support Program for High-Level Talents[2015R010] ; CAS Youth Innovation Promotion Association[2015144]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association
WOS SubjectChemistry, Medicinal
WOS Research AreaPharmacology & Pharmacy
WOS KeywordCHITOOLIGOSACCHARIDES ; IDENTIFICATION ; PURIFICATION ; EFFICIENT ; POLYMERIZATION ; OLIGOMERS ; MECHANISM ; GLYCANS
AbstractPartially acetylated chitosan oligosaccharides (COS), which consists of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) residues, is a structurally complex biopolymer with a variety of biological activities. Therefore, it is challenging to elucidate acetylation patterns and the molecular structure-function relationship of COS. Herein, the detailed deacetylation pattern of chitin deacetylase from Saccharomyces cerevisiae, ScCDA(2), was studied. Which solves the randomization of acetylation patterns during COS produced by chemical. ScCDA(2) also exhibits about 8% and 20% deacetylation activity on crystalline chitin and colloid chitin, respectively. Besides, a method for separating and detecting partially acetylated chitosan oligosaccharides by high performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS) system has been developed, which is fast and convenient, and can be monitored online. Mass spectrometry sequencing revealed that ScCDA(2) produced COS with specific acetylation patterns of DAAA, ADAA, AADA, DDAA, DADA, ADDA and DDDA, respectively. ScCDA(2) does not deacetylate the GlcNAc unit that is closest to the reducing end of the oligomer furthermore ScCDA(2) has a multiple-attack deacetylation mechanism on chitin oligosaccharides. This specific mode of action significantly enriches the existing limited library of chitin deacetylase deacetylation patterns. This fully defined COS may be used in the study of COS structure and function.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association
WOS IDWOS:000460795500002
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/165829
Collection中国科学院大连化学物理研究所
Corresponding AuthorYin, Heng
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
2.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China
3.Fujian Normal Univ, Coll Life Sci, Engn Res Ctr Ind Microbiol, Minist Educ, Fuzhou 350117, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Xian-Yu,Zhao, Yong,Zhang, Huai-Dong,et al. Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides[J]. MARINE DRUGS,2019,17(2):15.
APA Zhu, Xian-Yu,Zhao, Yong,Zhang, Huai-Dong,Wang, Wen-Xia,Cong, Hai-Hua,&Yin, Heng.(2019).Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides.MARINE DRUGS,17(2),15.
MLA Zhu, Xian-Yu,et al."Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides".MARINE DRUGS 17.2(2019):15.
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